TIMP-4 Antibody [Biotin]

• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TIMP-4 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIMP-4
  Cys30-Pro224
  Accession # Q99727
• Specificity: Detects human TIMP-4 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) TIMP-1, rhTIMP-2, rhTIMP-3, and recombinant mouse TIMP-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TIMP4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human TIMP-4 Antibody (Catalog # MAB974)
  ELISA Detection: Human TIMP-4 Biotinylated Antibody (Catalog # BAF974)
  Standard: Recombinant Human TIMP-4 (Catalog # 974-TSF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Tissue inhibitors of metalloproteinases (TIMPs) are a family of secreted proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four known members of the family, TIMP-1, -2, -3 and -4. TIMP-4 is produced by a wide range of tissues, particularly brain, heart, ovary and skeletal muscle (1, 2). Limited studies have shown that TIMP-4 has a tumor suppressive effect against Wilm’s tumor, exhibits negative correlation with glioma maligancy and is found in breast carcinoma cells (3 - 5). TIMP-4 inhibits MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, it binds to the hemopexin-like domain of pro-MMP-2 through its C-terminal domain in a manner similar to TIMP-2 (6). However, unlike TIMP-2, TIMP-4 does not promote pro-MMP-2 activation by MT1-MMP (MMP-14) (7). Although TIMP-4 is a potent inhibitor of most MMPs, it is not an effective inhibitor of ADAMs, such as TACE (8, 9).

1. Greene, et al. (1996) J. Biol. Chem. 271:30375.
2. Leco, et al. (1997) FEBS Lett. 401:213.
3. Geliker, et al. (2001) Oncogene 20:4337.
4. Groft, et al. (2001) Br. J. Cancer 85:55.
5. Hurst, et al. (2001) Biochem. Biophys. Res. Comm. 281:166.
6. Bigg, et al. (1997) J. Biol. Chem. 272:15496.
7. Hernandez-Barrantes, et al. (2001) Biochem. Biophys. Res. Comm. 281:126.
8. Amour, et al. (1998) FEBS Lett. 435:39.
9. Liu, et al. (1997) J. Biol. Chem. 272:20479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMP4