TIMP-2 Antibody (MM0035-2C13) - Azide and BSA Free

Immunohistochemistry-Paraffin: TIMP2 Antibody (MM0035-2C13) [NB110-61001] - Formalin fixed and paraffin embedded normal human placenta tissue is subjected to mouse anti human TIMP-2

• Reactivity: Hu
• Applications: WB, IHC
• Clone: MM0035-2C13
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TIMP-2 Antibody (MM0035-2C13) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TIMP-2 Antibody (MM0035-2C13) - Azide and BSA Free (NB110-61001) is a monoclonal antibody validated for use in IHC and WB. Anti-TIMP-2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant TIMP-2
• Specificity: No cross-reactivity to human TIMP-1 is noted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:100-1:1000
• Control:

  TIMP-2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for TIMP-2 Antibody (MM0035-2C13) - Azide and BSA Free

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. The activation and activity of MMPs are regulated by a family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP). TIMP2 (also called CSC-21K) is a 21 kDa glycoprotein that is expressed by a variety of cell types. It forms a non covalent, stoichiometric complex with both latent and active MMPs. TIMP2 shows a preference for MMP2. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP2 is constitutively produced in low (pg/ml) levels by most cell types in tissue culture. The low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-2 Antibody (NB110-61001)(1)

Publication using NB110-61001

• Perek B, Kowalska K et al. Variability in gelatinase expression in the walls of vessels used as aortocoronary conduits may impact long-term graft patency. Kardiol Pol 2019-01-25 [PMID: 30676640] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: TIMP2
• Entrez:
  • 7077(Human)
• Uniprot:
  • P16035()