The Quantikine Human TIMP-4 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TIMP-4 levels in cell culture supernates, serum, plasma, and human milk. It contains Sf 21-expressed recombinant human TIMP-4 and antibodies raised against the recombinant protein. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms. Natural human TIMP-4 showed dose-res...ponse curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human TIMP-4.
Natural and recombinant human TIMP-4. This kit detects recombinant human TIMP-4 in both free and MMP-bound forms.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Human TIMP-4 Quantikine ELISA Kit
metalloproteinase inhibitor 4
TIMP metallopeptidase inhibitor 4
tissue inhibitor of metalloproteinase 4
Tissue inhibitor of metalloproteinases 4
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.
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