TIMP-2 Antibody (F27 P3 A4)

Immunocytochemistry/Immunofluorescence: TIMP-2 Antibody (F27 P3 A4) [NB100-74552] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TIMP2 monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: TIMP-2 Antibody (F27 P3 A4) [NB100-74552] - Normal biopsies of deparaffinized Mouse pancreas tissue at a dilution of 1:100.

Immunocytochemistry/Immunofluorescence: TIMP-2 Antibody (F27 P3 A4) [NB100-74552] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TIMP2 monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunocytochemistry/Immunofluorescence: TIMP-2 Antibody (F27 P3 A4) [NB100-74552] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TIMP2 monoclonal antibody at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.

Immunohistochemistry-Paraffin: TIMP-2 Antibody (F27 P3 A4) [NB100-74552] - Normal biopsies of deparaffinized Human pancreas tissue at a dilution of 1:500.

• Reactivity: Hu, Mu, Rt, Bv, Ch, Rb
• Applications: WB, ICC/IF, IHC
• Clone: F27 P3 A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TIMP-2 Antibody (F27 P3 A4) Summary

• Immunogen: Synthetic peptide corresponding to residues D(56) S G N D I Y G N P I(66) of mouse TIMP-2.
• Localization: Secreted
• Predicted Species: Rat (100%), Chicken (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:500
  • Western Blot 1:500
• Application Notes: WB: Detects an approx. 18-24 kDa protein representing TIMP-2 from mouse platelets. It is recommended to block with 3% BSA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TIMP-2 Antibody (F27 P3 A4)

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) are inhibitory enzymes of matrix metalloproteinase family. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kD Type IV collagenase (MMP-2) and latent and active forms 92 kD Type IV collagenase (MMP-9). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMP2
• Entrez:
  • 21858(Mouse)
• Uniprot:
  • P25785(Mouse)