Mouse TIMP-1 ELISA Kit (Colorimetric) Summary
| Description |
Quality control test: Standard Curve. Timp1 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Timp1. |
| Sensitivity |
3 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
TIMP1 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
| Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
|
Components
|
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent.
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For standard/sample (cell culture medium) diluent.
- Detection Antibody TIMP-1 (Item F): 2 vial of biotinylated anti-mouse TIMP-1 (each vial is enough to assay half microplate).
- HRP-Streptavidin Concentrate (Item G): 8 µl 6,000x concentrated HRP-conjugated streptavidin.
- Standards (Item C): 2 vials of recombinant mouse TIMP-1.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TIMP-1 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-mouse TIMP-1.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse TIMP-1 ELISA Kit (Colorimetric)
Background
Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for TIMP-1 ELISA Kit (KA1814). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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