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HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for TIMP-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated) in the parental SK-OV-3 cell line, but is not detectable in knockout SK-OV-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND50 is typically 1 µg/mL." class="big_lightbox" target="_blank">
970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
980-MT) was serially diluted and captured by Rat Anti-Mouse TIMP‑1 Monoclonal Antibody (Catalog # MAB980) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF980) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.
![Western Blot TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP1_AF970_Western_Blot_19927.jpg)
![Immunohistochemistry TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/antibody/TIMP-1_AF980_Immunohistochemistry_7220.jpg)
![Immunocytochemistry TIMP-1 Antibody (63515) [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP1_MAB970_Immunocytochemistry__Immunofluorescence_23050.jpg)
![Sandwich ELISA TIMP-1 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/TIMP-1-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79290-img0001.jpg)
![N/A TIMP-1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_TIMP1_DTM100_ELISA_847.jpg)
![N/A TIMP-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_TIMP1_DY970_ELISA_2284.jpg)
![N/A TIMP-1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_TIMP1_MTM100_ELISA_1041.jpg)
![Western Blot TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP1_AF970_Western_Blot_21550.jpg)
![Immunohistochemistry TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP1_AF970_Immunohistochemistry_22978.jpg)
![Simple Western TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP1_AF970_Single_Cell_Western_21684.jpg)
![Knockout Validated TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP1_AF970_Knockout_Validated_22157.jpg)
![Neutralization TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP-1_AF970_Block_Neutralize_10044.jpg)
![Immunohistochemistry-Paraffin TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af970_human-timp-1-affinity-purified-polyclonal-ab-8220249503288.jpg)
![Proximity Ligation Assay TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af970_human-timp-1-affinity-purified-polyclonal-ab-82202410062.jpg)
![Proximity Ligation Assay TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af970_human-timp-1-affinity-purified-polyclonal-ab-44202415425028.jpg)
![ELISA TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/af970_human-timp-1-affinity-purified-polyclonal-ab-elisa-639201856336711886.jpg)
![Western Blot TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af980_mouse-timp-1-affinity-purified-polyclonal-ab-western-blot-121220259245223.jpg)
![ELISA TIMP-1 Antibody [Unconjugated]](http://images.novusbio.com/images/af980_mouse-timp-1-affinity-purified-polyclonal-ab-elisa-639201856336517861.jpg)
![Immunohistochemistry TIMP-1 Antibody (63515) [Unconjugated]](http://images.novusbio.com/images/antibody/TIMP-1_MAB970_Immunohistochemistry_7253.jpg)
![Western Blot TIMP-1 Antibody (63515) [Unconjugated]](http://images.novusbio.com/fullsize/mab970_human-timp-1-mab-clone-63515-western-blot-121220258175712.jpg)
![ELISA TIMP-1 Antibody (63515) [Unconjugated]](http://images.novusbio.com/images/mab970_human-timp-1-mab-clone-63515-elisa-639201781676658262.jpg)
![N/A TIMP-1 [HRP]](http://images.novusbio.com/fullsize/elisa/TIMP-1_DTM100_ELISA_194.jpg)
![N/A TIMP-1 [HRP]](http://images.novusbio.com/fullsize/elisa/TIMP-1_MTM100_ELISA_328.jpg)
