TIMP-1 Antibody (F31 P2 A5)

Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis was performed on whole cell extracts (30 ug lysate) of A431 (Lane 1), HeLa (Lane 2), Jurkat (Lane 3), HT29 (Lane 4) and COS-7 (Lane 5). The blots were probed with Anti-TIMP1 Mouse .

Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI.

Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Normal biopsies of deparaffinized Human prostate tissue at a dilution of 1:20.

Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis of TIMP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:20.

TGF-beta 1 induces the transformation from OF to MFB. (A) Western blot for TGF-beta 1 concentration and time; (B) Src and Acta2 expression in SRC gene knockdown group by q-PCR; and (C) the expression levels of alpha -SMA, Col-1, and Timp-1 in the SRC gene knockdown group by Western blot. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32457885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Signaling inhibitors. (A) Acta2 expression after Signaling inhibitors by q-PCR; (B) the expression of Smad3, Nfkb1, Pik3r1, and Akt1 in the SRC gene knockdown group by q-PCR; (C) the expression of alpha -SMA, Col-1, and Timp-1 after Signaling inhibitors by western blot; (D) the phosphorylation of SMAD2/3, NF-kappa B p65 and PI3K p85 proteins in SRC gene knockdown group by western blot. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32457885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

TGF-beta 1 induces the transformation from OF to MFB. (A) Western blot for TGF-beta 1 concentration and time; (B) Src and Acta2 expression in SRC gene knockdown group by q-PCR; and (C) the expression levels of alpha -SMA, Col-1, and Timp-1 in the SRC gene knockdown group by Western blot. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32457885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clone: F31 P2 A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TIMP-1 Antibody (F31 P2 A5) Summary

• Immunogen: Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.
• Localization: Secreted
• Specificity: TIMP-1 (F31 P2 A5)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunohistochemistry 1:20
  • Immunohistochemistry-Paraffin 1:10 - 1:100
  • Western Blot 1:250 - 1:1000
• Control:

  TIMP-1 Overexpression Lysate

Reactivity Notes

Rhesus monkey. Mouse reactivity reported in scientific literature (PMID: 19188864). Rat reactivity reported in scientific literature (PMID: 28275350).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TIMP-1 Antibody (F31 P2 A5)

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitor of metalloproteinases-1 (TIMP-1) and Tissue inhibitor of metalloproteinases-2 (TIMP-2) are inhibitory enzymes of matrix metalloproteinase family. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kD Type IV collagenase (MMP-2) and latent and active forms 92 kD Type IV collagenase (MMP-9). TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-1 Antibody (NB100-74551)(4)

Publications using NB100-74551

• Jurga AM, Piotrowska A, Makuch W et al. Blockade of P2X4 Receptors Inhibits Neuropathic Pain-Related Behavior by Preventing MMP-9 Activation and, Consequently, Pronociceptive Interleukin Release in a Rat Model. Front Pharmacol. 2017-03-09 [PMID: 28275350] (WB, Rat)
• Cao YL, Chen WL, Lei Q et al. The transplantation of rapamycin-treated senescent human mesenchymal stem cells with enhanced proangiogenic activity promotes neovascularization and ischemic limb salvage in mice Acta pharmacologica Sinica 2022-04-01 [PMID: 35365781] (WB, Human)
• Wu Y, Lu S, Huang X et al. Targeting cIAPs attenuates CCl4-induced liver fibrosis by increasing MMP9 expression derived from neutrophils Life sciences 2022-01-15 [PMID: 34914932] (WB, Mouse)

Bioinformatics

• Gene Symbol: TIMP1
• Entrez:
  • 7076(Human)