TIMP-1 Antibody (2A5)

Product Discontinued

TIMP-1 Antibody (2A5) Summary

• Immunogen: The antibody reacts with the TIMP-1 antigen.
• Specificity: The 2A5 monoclonal antibody was generated from mice immunized with a 11 mer-peptide derived from the sequence of human TIMP-1. The amino acid sequence of this peptide was: FQALGDAADIR.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Reactive with Human. The 11-mer peptide used for the immunization differs on at least 3 amino acids with the sequence of TIMP-1 of rat, mouse, and rabbit. Therefore it is not expected to be effective in these species as well. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Hybridoma culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Notes

For a positive control use Colon carcinoma.

Alternate Names for TIMP-1 Antibody (2A5)

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

Tissue inhibitor of metalloproteinase 1 (TIMP-1) is a stromal factor which forms complexes with various members of matrix metalloproteinases (MMPs) and irreversibly inactivates them. A multifunctional glycoprotein, TIMP-1 is one of the four members of TIMP family (TIMP 1-4) which exhibits 50% sequence similarity and expressed in wide variety of cells (1). TIMP-1 binds non-covalently in a 1:1 stoichiometric fashion to the active form of MMPs thereby inhibiting the proteolytic activity of these enzymes. Including inhibitory function, other studies have proposed growth protion, anti-apoptotic, anti- and pro-angiogenic functions for TIMP-1. TIMP-1 expression has been observed in poor clinical outcome in many cancer types, making it ideal prognostic marker in various cancer (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMP1