TIMM50 Antibody

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Western Blot: TIMM50 Antibody [NB100-1462] - NB100-1462 staining (0.5ug/ml) of Human Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TIMM50 Antibody Summary

Immunogen
Peptide with sequence CDVRTVLEHYALEDD corresponding to internal region according to NP_001001563.1.
Specificity
This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TIMM50
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Experiments gave bands at approx. 40 kDa band in human heart lysate after 0.5ug/ml antibody staining. This band corresponds to earlier findings in scientific literature with different antibodies scientific literature (PMID: 15044455). This protein has a calculated MW of 50.5 kDa band according to NP_001001563.
Publications
Read Publication using NB100-1462.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TIMM50 Antibody

  • homolog of yeast Tim50
  • MGC102733
  • mitochondrial import inner membrane translocase subunit TIM50
  • TIM50
  • TIM50L
  • Tim50-like protein
  • translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae)
  • translocase of inner mitochondrial membrane 50 homolog (yeast)

Background

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIMM50 Antibody (NB100-1462)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TIMM50
Entrez
Uniprot