TIGIT Antibody (741182) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human TIGIT PE-conjugated Monoclonal Antibody (Catalog # FAB7898P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 741182
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

TIGIT Antibody (741182) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIGIT
  Met1-Pro141
  Accession # Q495A1
• Specificity: Detects human TIGIT in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse TIGIT or recombinant human DNAM-1/CD226 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIGIT
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIGIT Antibody (741182) [Phycoerythrin]

• T cell immunoreceptor with Ig and ITIM domains
• TIGIT
• VSIG9
• VSTM3
• WUCAM

Background

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).

1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
5. SwissProt Accession # Q495A1.
6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
8. Joller, N. et al. (2011) J. Immunol. 83:1338.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIGIT Antibody (FAB7898P)(3)

Publications using FAB7898P

• Pascual-Pasto, G;McIntyre, B;Hines, MG;Giudice, AM;Garcia-Gerique, L;Hoffmann, J;Mishra, P;Matlaga, S;Lombardi, S;Shraim, R;Schürch, PM;Yarmarkovich, M;Hofmann, TJ;Alikarami, F;Martinez, D;Tsang, M;Gil-de-Gómez, L;Spear, TT;Bernt, KM;Wolpaw, AJ;Dimitrov, DS;Li, W;Bosse, KR; CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma Nature communications 2024-08-21 [PMID: 39164224] (Flow Cytometry, Human)
• T Pincikova, T Parrot, L Hjelte, M Högman, K Lisspers, B Ställberg, C Janson, A Malinovsch, JK Sandberg MAIT cell counts are associated with the risk of hospitalization in COPD Respiratory Research, 2022-05-18;23(1):127. 2022-05-18 [PMID: 35585629] (Flow Cytometry, Human)
• SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j J. Immunol., 2017-12-27;0(0):. 2017-12-27 [PMID: 29282306] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TIGIT
• Uniprot:
  • Q495A1(Human)