TIF1 gamma Antibody (6D1)

Western Blot: TIF1 gamma Antibody (6D1) [H00051592-M01] - TRIM33 monoclonal antibody (M01), clone 6D1. Analysis of TRIM33 expression in 293.

Immunocytochemistry/Immunofluorescence: TIF1 gamma Antibody (6D1) [H00051592-M01] - Analysis of monoclonal antibody to TRIM33 on HeLa cell. Antibody concentration 30 ug/ml.

Immunohistochemistry-Paraffin: TIF1 gamma Antibody (6D1) [H00051592-M01] - Analysis of monoclonal antibody to TRIM33 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: TIF1 gamma Antibody (6D1) [H00051592-M01] - Expression in PC-12.

Western Blot: TIF1 gamma Antibody (6D1) [H00051592-M01] - Expression in NIH/3T3.

Western Blot: TIF1 gamma Antibody (6D1) [H00051592-M01] - TRIM33 monoclonal antibody (M01), clone 6D1. Analysis of TRIM33 expression in HeLa.

Western Blot: TIF1 gamma Antibody (6D1) [H00051592-M01] - TRIM33 monoclonal antibody (M01), clone 6D1. Analysis of TRIM33 expression in Raw 264.7.

ELISA: TIF1 gamma Antibody (6D1) [H00051592-M01] - Detection limit for recombinant GST tagged TRIM33 is approximately 0.03ng/ml as a capture antibody.

Product Discontinued

TIF1 gamma Antibody (6D1) Summary

• Immunogen: TRIM33 (NP_056990, 1006 a.a. ~ 1105 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VKKKLQKKHSQHYQIPDDFVADVRLIFKNCERFNEMMKVVQVYADTQEINLKADSEVAQAGKAVALYFEDKLTEIYSDRTFAPLPEFEQEEDDGEVTEDS
• Localization: Nuclear
• Specificity: TRIM33 - tripartite motif-containing 33
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM33
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TIF1 gamma Antibody (6D1)

• E3 ubiquitin-protein ligase TRIM33
• EC 6.3.2
• EC 6.3.2.-
• Ectodermin homolog
• KIAA1113
• Protein Rfg7
• PTC7FLJ32925
• RET-fused gene 7 protein
• RFG7FLJ11429
• TF1G
• TIF1GAMMA
• TIF1-gamma
• TIF1GECTO
• TIFGAMMA
• Transcription intermediary factor 1-gamma
• transcriptional intermediary factor 1 gamma
• tripartite motif containing 33
• tripartite motif-containing 33
• Tripartite motif-containing protein 33

Background

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIF1 gamma Antibody (H00051592-M01)(3)

Publications using H00051592-M01

• Pinal-Fernandez I, Ferrer-Fabregas B, Trallero-Araguas E et al. Tumour TIF1 mutations and loss of heterozygosity related to cancer-associated myositis. Rheumatology (Oxford) 2017-11-14 [PMID: 29149307]
• Ding ZY, Jin GN, Wang W et al. Reduced expression of TIF1gamma promotes metastasis and indicates poor prognosis of hepatocellular carcinoma. Hepatology. 2014-09-25 [PMID: 24954480]
• Fiorentino DF, Chung LS, Christopher-Stine L et al. Most Patients With Cancer-Associated Dermatomyositis Have Antibodies to Nuclear Matrix Protein NXP-2 or Transcription Intermediary Factor 1gamma. Arthritis Rheum. 2013-11-01 [PMID: 24037894] (WB, Human)

Bioinformatics

• Gene Symbol: TRIM33
• Entrez:
  • 51592(Human)
• OMIM:
  • 188550(Human)
• Uniprot:
  • NP_056990(Human)