Tie-2 Antibody (83715) [Allophycocyanin]

HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human Tie-2 APC-conjugated Monoclonal Antibody (Catalog # FAB3131A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 83715
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Tie-2 Antibody (83715) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Tie‑2
  Ala23-Lys745
  Accession # AAA61139
• Specificity: Detects human Tie-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Tie‑1, recombinant mouse Tie-2, or recombinant zebrafish Tie-2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TEK
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-2 Antibody (83715) [Allophycocyanin]

• angiopoietin-1 receptor
• CD202b antigen
• CD202b
• EC 2.7.10
• EC 2.7.10.1
• hTIE2
• p140 TEK
• soluble TIE2 variant 1
• soluble TIE2 variant 2
• TEK tyrosine kinase, endothelial
• TEK
• Tie2
• Tie-2
• TIE2CD202b
• Tunica interna endothelial cell kinase
• Tyrosine-protein kinase receptor TEK
• Tyrosine-protein kinase receptor TIE-2
• venous malformations, multiple cutaneous and mucosal
• VMCM
• VMCM1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
2. Takakura, N. et al. (1998) Immunity 9:677.
3. Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-2 Antibody (FAB3131A)(12)

Publications using FAB3131A

• Otani, Y;Schol, J;Sakai, D;Nakamura, Y;Sako, K;Warita, T;Tamagawa, S;Ambrosio, L;Munesada, D;Ogasawara, S;Matsushita, E;Kawachi, A;Naiki, M;Sato, M;Watanabe, M; Assessment of Tie2-Rejuvenated Nucleus Pulposus Cell Transplants from Young and Old Patient Sources Demonstrates That Age Still Matters International journal of molecular sciences 2024-07-30 [PMID: 39125917] (Flow Cytometry, Human)
• Tamagawa, S;Sakai, D;Nojiri, H;Nakamura, Y;Warita, T;Matsushita, E;Schol, J;Soma, H;Ogasawara, S;Munesada, D;Koike, M;Shimizu, T;Sato, M;Ishijima, M;Watanabe, M; SOD2 orchestrates redox homeostasis in intervertebral discs: A novel insight into oxidative stress-mediated degeneration and therapeutic potential Redox biology 2024-02-19 [PMID: 38412803] (Flow Cytometry, Human)
• Stephane Oudard, Nadine Benhamouda, Bernard Escudier, Patrice Ravel, Thi Tran, Emeline Levionnois et al. Decrease of Pro-Angiogenic Monocytes Predicts Clinical Response to Anti-Angiogenic Treatment in Patients with Metastatic Renal Cell Carcinoma Cells 12/22/2021 [PMID: 35011579]
• M Adjuto-Sac, P Soubeyran, J Garcia, S Audebert, L Camoin, M Rubis, J Roques, B Binétruy, JL Iovanna, R Tournaire TNF-&alpha induces endothelial-mesenchymal transition promoting stromal development of pancreatic adenocarcinoma Cell Death & Disease, 2021-06-25;12(7):649. 2021-06-25 [PMID: 34172716] (Flow Cytometry, Human)
• Daisuke Sakai, Jordy Schol, Frances C. Bach, Adel Tekari, Nobuho Sagawa, Yoshihiko Nakamura et al. Successful fishing for nucleus pulposus progenitor cells of the intervertebral disc across species JOR SPINE 6/27/2018 [PMID: 31463445]
• Rita Campanelli Tie2 Expressing Monocytes in the Spleen of Patients with Primary Myelofibrosis PLoS ONE, 2016-06-09;11(6):e0156990. 2016-06-09 [PMID: 27281335] (Flow Cytometry, Human)
• Xinjing Wang, Zhiyuan Dai, Xiaoli Wu, Kai Wang, Xipeng Wang Distinct RNA transcriptome patterns are potentially associated with angiogenesis in Tie2-expressing monocytes Gene 4/10/2016 [PMID: 26748243]
• Yi-Feng He, Chao-Qun Wang, Yao Yu, Jing Qian, Kang Song, Qi-Man Sun et al. Tie2-Expressing Monocytes Are Associated with Identification and Prognoses of Hepatitis B Virus Related Hepatocellular Carcinoma after Resection PLOS ONE 11/23/2015 [PMID: 26599011]
• Fiorio Pla , Alessand, Brossa , Alessia, Bernardini , Michela, Genova , Tullio, Grolez , Guillaum, Villers , Arnaud, Leroy , Xavier, Prevarskaya , Natalia, Gkika , Dimitra, Bussolati , Benedett Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib. BMC Cancer, 2014-12-12;14(0):939. 2014-12-12 [PMID: 25494980] (Flow Cytometry, Human)
• Ebtisam Al-ofi, Seth B. Coffelt, Dilly O. Anumba Monocyte Subpopulations from Pre-Eclamptic Patients Are Abnormally Skewed and Exhibit Exaggerated Responses to Toll-Like Receptor Ligands PLoS ONE 7/27/2012 [PMID: 22848746]
• Wang C, Tang X, Sun X, Miao Z, Lv Y, Yang Y, Zhang H, Zhang P, Liu Y, DU L, Gao Y, Yin M, Ding M, Deng H TGFbeta inhibition enhances the generation of hematopoietic progenitors from human ES cell-derived hemogenic endothelial cells using a stepwise strategy. Cell Res., 2011-08-23;22(1):194-207. 2011-08-23 [PMID: 21862970] (Flow Cytometry, Human)
• Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology. J. Thromb. Haemost., 2011-05-01;9(5):1056-66. 2011-05-01 [PMID: 21342432] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TEK
• Uniprot:
  • AAA61139()