TIAM1 Antibody - BSA Free

Immunohistochemistry: TIAM1 Antibody [NBP3-35111] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TIAM1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: TIAM1 Antibody [NBP3-35111] - Western blot analysis of lysates from Rat testis, using TIAM1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.

Immunohistochemistry: TIAM1 Antibody [NBP3-35111] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TIAM1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TIAM1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TIAM1 Antibody - BSA Free (NBP3-35111) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1392-1591 of human TIAM1 (NP_003244.2).
  
  Sequence:
  SILRDKHRRQLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAVSASSPEKESQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESVKGASVDRDLQERLQATSISQRERGRKTLDSHASRMAQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIAM1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 178 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TIAM1 Antibody - BSA Free

• FLJ36302
• human T-lymphoma invasion and metastasis inducing TIAM1 protein, 10TIAM-1
• T-cell lymphoma invasion and metastasis 1
• TIAM1
• T-lymphoma invasion and metastasis-inducing protein 1

Background

TIAM1 modulates the activity of RHO like proteins and connects extracellular signals to cytoskeletal activities. It acts as a GDP dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO like GTPases and activates them. It activates RAC1, CDC42, and to a lesser extent RHOA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIAM1