TIAM1 Antibody

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TIAM1 Antibody Summary

• Description: The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
• Immunogen: Human Tiam1 (catalytic domain/ C-terminal domain) is an affinity purified rabbit polyclonal antiserum derived by injection of rabbits with a GST fusion protein with the C-terminal amino acids 904-1511 of Tiam 1.
• Specificity: Human Tiam1 (catalytic domain/ C-terminal domain) recognizes the C-terminal amino acids 904-1511 of Tiam 1 containing the DH-PH regions.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIAM1
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Rabbit antiserum
• Preservative: 0.09% Sodium Azide
• Purity: Whole Antiserum

Alternate Names for TIAM1 Antibody

• FLJ36302
• human T-lymphoma invasion and metastasis inducing TIAM1 protein, 10TIAM-1
• T-cell lymphoma invasion and metastasis 1
• TIAM1
• T-lymphoma invasion and metastasis-inducing protein 1

Background

TIAM1 modulates the activity of RHO like proteins and connects extracellular signals to cytoskeletal activities. It acts as a GDP dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO like GTPases and activates them. It activates RAC1, CDC42, and to a lesser extent RHOA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIAM1