TIA1 Antibody

Western Blot: TIA1 Antibody [NBP1-33452] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: TIA1 Antibody [NBP1-33452] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TIA1 stained by TIA1 antibody [N1N3] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10um.

Product Discontinued

TIA1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TIA1. The exact sequence is proprietary.
• Localization: Cytoplasmic granule
• Predicted Species: Mouse (100%), Rat (90%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 43 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (83%), Xenopus laevis (88%), Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for TIA1 Antibody

• cytotoxic granule-associated RNA-binding protein
• nucleolysin TIA-1 isoform p40
• p40-TIA-1 (containing p15-TIA-1)
• p40-TIA-1
• RNA-binding protein TIA-1
• T-cell-restricted intracellular antigen-1
• TIA1 cytotoxic granule-associated RNA binding protein
• TIA1 cytotoxic granule-associated RNA-binding protein
• TIA-1

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TIA1