TIA1 Antibody (TIA1/1352R)

Immunohistochemistry-Paraffin: TIA1 Antibody (TIA1/1352R) [NBP3-13791] - IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using TIA1 antibody (TIA1/1352R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: TIA1 Antibody (TIA1/1352R) [NBP3-13791] - Formalin-fixed, paraffin-embedded human spleen stained with TIA1 antibody (TIA1/1352R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: TIA1 Antibody (TIA1/1352R) [NBP3-13791] - Formalin-fixed, paraffin-embedded human lymph node stained with TIA1 antibody (TIA1/1352R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: TIA1 Antibody (TIA1/1352R) [NBP3-13791] - Formalin-fixed, paraffin-embedded human tonsil stained with TIA1 antibody (TIA1/1352R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Product Discontinued

TIA1 Antibody (TIA1/1352R) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Human bone marrow malignant cells from a non B, non T acute leukemia
• Localization: Cytoplasmic granule, Nucleus, Accumulates in cytoplasmic stress granules (SG) following cellular damage
• Specificity: The T cell intracellular antigen 1 (TIA 1) is a 17 kDa cytoplasmic granule associated protein also designated as GMP 17, for granule membrane protein of 17 kDa. The GMP 17/TIA 1 molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95) mediated apoptosis. Within hematopoietic cell lines, the TIA1/1352R monoclonal antibody reacts with about 90% of CD16+, 50 60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T cell clones, activated NK cell clones, and Con activated thymocytes, but not with B lymphocytes or B cell lines. TIA1 antibody labels cytotoxic T cells and natural killer cells (NK cells). It is also expressed in T-cell lymphoma, large granular lymphocyte (LGL) leukemia and hairy cell leukemia. TIA1 expression in T-cell malignancies may help in differentiating LGL leukemia (high expression) from T-cell lymphocytosis and other T-cell diseases (low expression). TIA1 may also be used to label tumor-infiltrating lymphocytes in the study of immune response to malignancies.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TIA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Notes

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-14194)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for TIA1 Antibody (TIA1/1352R)

• cytotoxic granule-associated RNA-binding protein
• nucleolysin TIA-1 isoform p40
• p40-TIA-1 (containing p15-TIA-1)
• p40-TIA-1
• RNA-binding protein TIA-1
• T-cell-restricted intracellular antigen-1
• TIA1 cytotoxic granule-associated RNA binding protein
• TIA1 cytotoxic granule-associated RNA-binding protein
• TIA-1

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIA1
• Uniprot:
  • P31483(Human)