Thymidylate Synthase Antibody (TYMS/1884)

Images

 
Western Blot: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Western Blot Analysis of human HeLa cell lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Immunocytochemistry/ Immunofluorescence: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with Thymidylate Synthase Mouse Monoclonal Antibody ...read more
Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).
Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Flow Cytometric Analysis of PFA-fixed Ramos cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti- Mouse ...read more
Flow Cytometry: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Flow Cytometric Analysis of PFA-fixed MOLT4 cells. Thymidylate Synthase Monospecific Mouse Monoclonal Antibody followed by goat anti- Mouse ...read more
Protein Array: Thymidylate Synthase Antibody (TYMS/1884) [NBP3-07144] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase Monospecific Mouse Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PA
Clone
TYMS/1884
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Thymidylate Synthase Antibody (TYMS/1884) Summary

Immunogen
Recombinant human Thymidylate Synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
Localization
Nuclear & Cytoplasmic
Isotype
IgG2c Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TYMS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08882)

Antibody with azide - store at 2 to 8C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Thymidylate Synthase Antibody (TYMS/1884)

  • EC 2.1.1.45
  • HsT422
  • MGC88736
  • thymidylate synthase
  • thymidylate synthetase
  • TMSTsase
  • TSase
  • TSHST422

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TYMS