Thymidylate Synthase Antibody (SPM453)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM453
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Thymidylate Synthase Antibody (SPM453) Summary

Immunogen
Recombinant human thymidylate synthase
Localization
Cytoplasmic
Marker
5-FU Resistance Marker
Specificity
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TYMS
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug
Application Notes
ELISA (For coating, order NBP2-34441 (BSA free version of NBP2-34390))
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-34390 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Thymidylate Synthase Antibody (SPM453)

  • EC 2.1.1.45
  • HsT422
  • MGC88736
  • thymidylate synthase
  • thymidylate synthetase
  • TMSTsase
  • TSase
  • TSHST422

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thymidylate Synthase Antibody (NBP2-34390) (0)

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Review for Thymidylate Synthase Antibody (NBP2-34390) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-34390:
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Images Ratings Applications Species Date Details
Western Blot Thymidylate Synthase NBP2-34390
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reviewed by:
Masashi Okada
WB Human 09/14/2017
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Summary

ApplicationWestern Blot
Sample TestedTumor cell lyastes
SpeciesHuman
Lot7298-1XP 160623

Comments

Commentsthree tumor cell lysates (20 ug) are loaded.
1/1000 dil Ab in 2% BSA/TBST, incubated for o/n at 4C

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Thymidylate Synthase Antibody (NBP2-34390) (0)

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Secondary Antibodies

 

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Recent Reviews

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1
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Masashi Okada
09/14/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TYMS
Uniprot