Thymidylate Synthase Antibody (SPM453)

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Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase Mouse Monoclonal Antibody (SPM453).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clone
SPM453
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Thymidylate Synthase Antibody (SPM453) Summary

Immunogen
Recombinant full-length human Thymidylate Synthase protein. (Uniprot: P04818 )
Localization
Cytoplasm, Nucleus, Mitochondria
Marker
5-FU Resistance Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TYMS
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-34390 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34441)

Antibody with azide - store at 2 to 8C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Thymidylate Synthase Antibody (SPM453)

  • EC 2.1.1.45
  • HsT422
  • MGC88736
  • OK/SW-cl.29
  • Thymidylate Synthase
  • thymidylate synthetase
  • TMSTsase
  • TS
  • TSase
  • TSHST422
  • TYMS

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thymidylate Synthase Antibody (NBP2-34390) (0)

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Review for Thymidylate Synthase Antibody (NBP2-34390) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34390:
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Images Ratings Applications Species Date Details
Western Blot Thymidylate Synthase NBP2-34390
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3
reviewed by:
Verified Customer
WB Human 09/14/2017
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Summary

ApplicationWestern Blot
Sample TestedTumor cell lyastes
SpeciesHuman
Lot7298-1XP 160623

Comments

Commentsthree tumor cell lysates (20 ug) are loaded.
1/1000 dil Ab in 2% BSA/TBST, incubated for o/n at 4C

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Thymidylate Synthase Antibody (NBP2-34390) (0)

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Verified Customer
09/14/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TYMS
Uniprot