Thymidylate Synthase Antibody (SPM453)

Images

 
Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (SPM453).
Flow (Intracellular): Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - An intracellular stain was performed on Hek293 cells with Thymidylate Synthase Antibody [SPM453] NBP2-34441PE (blue) and a matched isotype ...read more
Flow (Intracellular): Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - An intracellular stain was performed on HEK293 cells with Thymidylate Synthase Antibody [SPM453] NBP2-34441PE (blue) and a matched isotype ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
SPM453
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Thymidylate Synthase Antibody (SPM453) Summary

Immunogen
Recombinant human thymidylate synthase
Localization
Cytoplasmic
Marker
5-FU Resistance Marker
Specificity
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TYMS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-0.5ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug
  • Flow (Intracellular)
Application Notes
ELISA (For coating, order NBP2-34441 (BSA free version of NBP2-34390))
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-34390 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Thymidylate Synthase Antibody (SPM453)

  • EC 2.1.1.45
  • HsT422
  • MGC88736
  • thymidylate synthase
  • thymidylate synthetase
  • TMSTsase
  • TSase
  • TSHST422

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thymidylate Synthase Antibody (NBP2-34390) (0)

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Review for Thymidylate Synthase Antibody (NBP2-34390) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-34390:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Thymidylate Synthase NBP2-34390
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reviewed by:
Masashi Okada
WB Human 09/14/2017
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Summary

ApplicationWestern Blot
Sample TestedTumor cell lyastes
SpeciesHuman
Lot7298-1XP 160623

Comments

Commentsthree tumor cell lysates (20 ug) are loaded.
1/1000 dil Ab in 2% BSA/TBST, incubated for o/n at 4C

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Thymidylate Synthase Antibody (NBP2-34390) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34441AF350
Alexa Fluor 405 NBP2-34441AF405
Alexa Fluor 488 NBP2-34441AF488
Alexa Fluor 532 NBP2-34441AF532
Alexa Fluor 594 NBP2-34441AF594
Alexa Fluor 647 NBP2-34441AF647
Alexa Fluor 700 NBP2-34441AF700
Alexa Fluor 750 NBP2-34441AF750
Allophycocyanin NBP2-34441APC
Biotin NBP2-34441B
DyLight 350 NBP2-34441UV
DyLight 405 NBP2-34441V
DyLight 488 NBP2-34441G
DyLight 550 NBP2-34441R
DyLight 594 NBP2-34441DL594
DyLight 650 NBP2-34441C
DyLight 680 NBP2-34441FR
DyLight 755 NBP2-34441IR
FITC NBP2-34441F
HRP NBP2-34441H
Janelia Fluor 549 NBP2-34441JF549
Janelia Fluor 646 NBP2-34441JF646
PE NBP2-34441PE
PerCP NBP2-34441PCP

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Recent Reviews

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1
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Masashi Okada
09/14/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TYMS
Uniprot