Thymidine Kinase 1 Antibody (JF0970)

Western Blot: Thymidine Kinase 1 Antibody (JF0970) [NBP2-67199] - Analysis of Thymidine Kinase 1 on different lysates using anti-Thymidine Kinase 1 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Hela

Immunocytochemistry/Immunofluorescence: Thymidine Kinase 1 Antibody (JF0970) [NBP2-67199] - Staining Thymidine Kinase 1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Thymidine Kinase 1 Antibody (JF0970) [NBP2-67199] - Analysis of paraffin-embedded human tonsil tissue using anti-Thymidine Kinase 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Thymidine Kinase 1 Antibody (JF0970) [NBP2-67199] - Analysis of Hela cells with Thymidine Kinase 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: Thymidine Kinase 1 Antibody (JF0970) [NBP2-67199] - Staining Thymidine Kinase 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Thymidine Kinase 1 Antibody (JF0970) [NBP2-67199] - Staining Thymidine Kinase 1 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Thymidine Kinase 1 Antibody (JF0970) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Thymidine Kinase 1 aa 2-49 / 234. (SwissProt: P04183 Human; SwissProt: P04184 Mouse)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Thymidine Kinase 1 Antibody (JF0970)

• EC 2.7.1.21
• thymidine kinase 1 soluble isoform
• Thymidine Kinase 1
• thymidine kinase 1, soluble
• thymidine kinase, cytosolic
• thymidine kinase-1
• TK1
• TK2

Background

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TK1