Thymidine Kinase 1 Antibody (1008515) [DyLight 755]

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1008515
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 755

Thymidine Kinase 1 Antibody (1008515) [DyLight 755] Summary

• Immunogen: E. coli-derived recombinant human Thymidine Kinase 1
  Met1-Asn234
  Accession # P04183
• Specificity: Detects human Thymidine Kinase 1 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Thymidine Kinase 1 Antibody (1008515) [DyLight 755]

• EC 2.7.1.21
• thymidine kinase 1 soluble isoform
• Thymidine Kinase 1
• thymidine kinase 1, soluble
• thymidine kinase, cytosolic
• thymidine kinase-1
• TK1
• TK2

Background

Thymidine kinase (TK) belongs to a group of enzymes such as dihydrofolate reductase, thymidylate synthase, and DNA polymerase that are involved in DNA synthesis and precursor production. Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells due to multiple regulatory mechanisms that ensure expression of these enzymes exclusively in replicating cells. TK is responsible for catalyzing the phosphorylation of thymidine, which functions as a part of the pyrimidine salvage pathway involved in DNA synthesis. The activities of enzymes such as TK are essential for the activation of several chemotherapeutically important nucleoside analogues that are administered as prodrugs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.