THSD1 Antibody

Western Blot: THSD1 Antibody [NBP1-86930] - Analysis in control (vector only transfected HEK293T lysate) and THSD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: THSD1 Antibody [NBP1-86930] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: THSD1 Antibody [NBP1-86930] - Staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

THSD1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VPPEDDASGSESFQSNAQKIIPPLFSYRLAQQQLKEMKKKGLTETTKVYHVSQSPLTDTAIDAAPSAPLDLESPEEAAANKFRIKSPFPEQPAVSAGERPPSRLDLNVTQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THSD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 28576485). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  THSD1 Protein (NBP1-86930PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for THSD1 Antibody

• thrombospondin type-1 domain-containing protein 1
• thrombospondin, type I, domain 1
• thrombospondin, type I, domain containing 1,4833423O18Rik
• THSD1
• TMTSP
• TMTSPMGC74971
• Transmembrane molecule with thrombospondin module
• UNQ3010

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for THSD1 Antibody (NBP1-86930)(1)

Publication using NBP1-86930

• Xu Z, Rui YN, Balzeau J et al. Highly efficient one-step scarless protein tagging by type IIS restriction endonuclease-mediated precision cloning Biochem. Biophys. Res. Commun. 2017-08-12 [PMID: 28576485] (WB, Human)

Bioinformatics

• Gene Symbol: THSD1