Thrombospondin-4 Antibody (276523)

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thrombospondin-4 Monoclonal Antibody (Catalog # MAB2390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

The picture of CAFs and NFs stained with Podoplanin and the western blot analysis of THBS4.(A), Representative picture of Podoplanin staining. Podoplanin was mainly stained at the cytoplasm fibroblasts. The expression level of Podoplanin was higher in CAFs, in compared with that in NFs. (B), THBS4 expression. CAFs expressed THBS4, but NFs and cancer cells did not. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31703077), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clone: 276523
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Thrombospondin-4 Antibody (276523) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
  Ala22-Asn961 (Pro276Ala, Ala420Val)
  Accession # P35443
• Specificity: Detects human Thrombospondin-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human Thrombospondin-1, -2, or -3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: THBS4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombospondin-4 Antibody (276523)

• THBS4
• thrombospondin 4
• Thrombospondin4
• Thrombospondin-4
• TSP4
• TSP-4

Background

Thrombospondin-4 (TSP-4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. TSP‑1 and -2 constitute subgroup A and form disulfide-linked homotrimers, whereas TSP-3, -4, and -5/COMP constitute subgroup B and form pentamers (1, 2). The human TSP-4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven TSP type-3 repeats (one with an RGD motif), and a TSP C-terminal domain (3). Human TSP-4 shares 93% aa sequence identity with mouse and rat TSP-4. Within the TSP type-3 repeats and the TSP C-terminal domain, human TSP-4 shares 79% aa sequence identity with TSP-3 and COMP, and 58% aa sequence identity with TSP-1 and -2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). TSP-4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of TSP-4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP-4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). TSP-4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of TSP-4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type TSP-4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type TSP-4, although the A387P variant induces a greater release of proinflammatory molecules (13).

1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
9. Stenina, O.I. et al. (2003) Circulation 108:1514.
10. Topol, E.J. et al. (2001) Circulation 104:2641.
11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
13. Pluskota, E. et al. (2005) Blood 106:3970.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thrombospondin-4 Antibody (MAB2390)(4)

Publications using MAB2390

• K Kuroda, M Yashiro, T Sera, Y Yamamoto, Y Kushitani, A Sugimoto, S Kushiyama, S Nishimura, S Togano, T Okuno, T Tamura, T Toyokawa, H Tanaka, K Muguruma, M Ohira The clinicopathological significance of Thrombospondin-4 expression in the tumor microenvironment of gastric cancer PLoS ONE, 2019-11-08;14(11):e0224727. 2019-11-08 [PMID: 31703077] (IHC, Western Blot, Human)
• B Lana, KM Page, I Kadurin, S Ho, M Nieto-Rost, AC Dolphin Thrombospondin-4 reduces binding affinity of [(3)H]-gabapentin to calcium-channel ?2?-1-subunit but does not interact with ?2?-1 on the cell-surface when co-expressed Sci Rep, 2016-04-14;6(0):24531. 2016-04-14 [PMID: 27076051] (Western Blot, Human)
• Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling. Mod. Pathol., 2011-06-24;24(10):1390-403. 2011-06-24 [PMID: 21701537] (IHC-Fr, Human)
• Greco SA, Chia J, Inglis KJ Thrombospondin-4 is a putative tumour-suppressor gene in colorectal cancer that exhibits age-related methylation. BMC Cancer, 2010-09-16;10(0):494. 2010-09-16 [PMID: 20846368] (IHC-P, Human)

Review for Thrombospondin-4 Antibody (MAB2390) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 02/19/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 23942617
• Species: Human

Bioinformatics

• Gene Symbol: THBS4
• Uniprot:
  • P35443()