Human Thrombospondin-1 ELISA Kit (Colorimetric)

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ELISA: Human Thrombospondin-1 ELISA Kit (Colorimetric) [NBP2-60149] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, breast milk, saliva, tissue homogenates, cell culture supernates
Standard Curve Range
2.7 ng/ml-2000 ng/ml
Sensitivity
2.7 ng/ml

Order Details

Human Thrombospondin-1 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human TSP-1. No significant cross-reactivity or interference between human TSP-1 and analogues was observed.
Standard Curve Range
2.7 ng/ml-2000 ng/ml
Sensitivity
2.7 ng/ml
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)
Gene
THBS1

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Instruction manual
  2. HRP-avidin (100 x concentrate)
  3. HRP-avidin Diluent
  4. Assay plate (12 x 8 coated Microwells)
  5. Biotin-antibody (100 x concentrate)
  6. Biotin-antibody Diluent
  7. Adhesive Strip (For 96 wells)
  8. TMB Substrate
  9. Wash Buffer (25 x concentrate)
  10. Stop Solution
  11. Standard (Freeze dried)
  12. Sample Diluent

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Human Thrombospondin-1 ELISA Kit (Colorimetric)

  • THBS
  • THBS1
  • THBS-1
  • thrombospondin 1
  • Thrombospondin1
  • Thrombospondin-1
  • thrombospondin-1p180
  • TSP-1
  • TSP1thrombospondin-1
  • TSPTSP-1

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol THBS1