Thrombopoietin R/Tpo R Antibody [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

Thrombopoietin R/Tpo R Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin R/Tpo R
  Ser18-Trp483
  Accession # CAA52031
• Specificity: Detects mouse Thrombopoietin R/Tpo R in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Tpo R is observed and less than 2% cross-reactivity with recombinant mouse Epo R is observed
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombopoietin R/Tpo R Antibody [Alexa Fluor® 405]

• CD110 antigen
• CD110
• c-Mpl
• MPL
• MPLV
• Myeloproliferative leukemia protein
• myeloproliferative leukemia virus oncogene
• Proto-oncogene c-Mpl
• Thrombopoietin R
• thrombopoietin receptor
• TpoR
• TPO-R
• TPORMPLV

Background

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a type I membrane protein that is a member of the hematopoietin/cytokine receptor superfamily. Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. Receptor dimerization occurs upon ligand binding. Downstream signaling cascades utilize the Ras/MAP and JAK/STAT pathways (1). In humans, variations at the 3’ end on the molecule result in the formation of 3 distinct mRNA species, a P-form, a K-form, and a potentially secreted form. The predominant form in humans is the P-form, which encodes a full length receptor. In mouse, mRNA for a full length form, a potentially secreted form, and a form lacking 8 amino acids in the extra-cytoplasmic domain has been detected (2). The human and mouse receptors share approximately 81% amino acid identity. Tpo R is expressed at low levels in various cell types including hematopoietic progenitor cells, megakaryocytes and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.