Thrombomodulin/BDCA-3 Antibody (JB48-23)

Western Blot: Thrombomodulin/BDCA-3 Antibody (JB48-23) [NBP2-75683] - Analysis of Thrombomodulin on A431 cell using anti-Thrombomodulin antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (JB48-23) [NBP2-75683] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Thrombomodulin/BDCA-3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (JB48-23) [NBP2-75683] - Analysis of paraffin-embedded human placenta tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (JB48-23) [NBP2-75683] - Analysis of paraffin-embedded human lung cancer tissue using anti-Thrombomodulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (JB48-23) [NBP2-75683] - Analysis of paraffin-embedded human lung tissue with Rabbit anti-Thrombomodulin/BDCA-3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Thrombomodulin/BDCA-3 Antibody (JB48-23) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Thrombomodulin/BDCA-3 aa 526-575 / 575. (SwissProt: P07204 Human)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: THBD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Thrombomodulin/BDCA-3 Antibody (JB48-23)

• BDCA3
• BDCA-3
• CD141 antigen
• CD141
• Fetomodulin
• THBD
• THRM
• Thrombomodulin
• TM

Background

CD141/Thrombomodulin is an exclusively endothelial cell surface glycoprotein that forms a 1:1 complex with thrombin. Binding of thrombin to this high affinity receptor alters its specificity toward several substrates. The complex activates protein C approximately 1000 times faster than thrombin alone. Activated protein C degrades clotting factors V and VIII; thus, thrombomodulin converts thrombin into a physiologic anticoagulant. Thrombomodulin is also found in the circulatory and urinary systems, the physiologic significance of this is obscure.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THBD