Themis Antibody

Western Blot: THEMIS Antibody [NBP2-27492] - (0.3 ug/ml) Analysis of Mouse Spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Themis Antibody Summary

• Description: Novus Biologicals Goat Themis Antibody (NBP2-27492) is a polyclonal antibody validated for use in WB and ELISA. Anti-Themis Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EERTINLPKSLKSH corresponding to C-Terminus according to NP_848781.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: THEMIS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 72 kDa band observed in mouse spleen lysates (calculated MW of 72.7 kDa band according to NP_848781.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Themis Antibody

• bA325O24.3
• bA325O24.4
• C6orf190
• C6orf207
• FLJ40584
• Gasp
• GRB2-associated protein
• MGC163388
• protein THEMIS
• signaling phosphoprotein specific for T cells
• SPOT
• Themis
• thymocyte selection associated
• thymocyte selection pathway associated
• thymocyte-expressed molecule involved in selection
• TSEPA

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Themis Antibody (NBP2-27492)(1)

Publication using NBP2-27492

• Lesourne R, Uehara S, Lee J et al. Themis, a T cell-specific protein important for late thymocyte development. Nat Immunol 2009-08-01 [PMID: 19597498]

Bioinformatics

• Gene Symbol: THEMIS
• Entrez:
  • 210757(Mouse)
• Uniprot:
  • NP_848781.1(Human)