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Themis Antibody (719945) [Alexa Fluor® 350]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
719945
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 350
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
IC6816V-100UG
Alexa Fluor 488
IC6816G-100UG
Alexa Fluor 594
IC6816T-100UG
Alexa Fluor 647
IC6816R-100UG
Alexa Fluor 700
IC6816N-100UG
Alexa Fluor 750
IC6816S-100UG
Unconjugated
MAB6816
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB6816
Carrier Free
MAB6816
Carrier Free
MAB6816-SP
Carrier Free
IC6816T-100UG
Carrier Free
IC6816S-100UG
Carrier Free
IC6816R-100UG
Carrier Free
IC6816N-100UG
Carrier Free
IC6816V-100UG
Carrier Free
IC6816G-100UG
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Themis Antibody (719945) [Alexa Fluor® 350] Summary

Immunogen
E. coli-derived recombinant mouse Themis
Lys122-Lys237
Accession # Q8BGW0
Specificity
Detects mouse Themis in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2‑282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2‑282) is observed under reducing conditions.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
THEMIS
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Themis Antibody (719945) [Alexa Fluor® 350]

  • bA325O24.3
  • bA325O24.4
  • C6orf190
  • C6orf207
  • FLJ40584
  • Gasp
  • GRB2-associated protein
  • MGC163388
  • protein THEMIS
  • signaling phosphoprotein specific for T cells
  • SPOT
  • Themis
  • thymocyte selection associated
  • thymocyte selection pathway associated
  • thymocyte-expressed molecule involved in selection
  • TSEPA

Background

Themis (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, mouse Themis shares 87% and 96% aa sequence identity with human and rat Themis, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: GPRASP1 Antibody [NBP2-81951] - Analysis of GPRASP1 in human breast carcinoma tissue with GPRASP1 antibody at 5 ug/ml.
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Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
(2 Reviews)
(16 Publications)
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Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Src [p Tyr419]
Antibody [Unconjugate
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human colon using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(1 Review)
(5 Publications)
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Applications: WB, Simple Western, IHC
C-Reactive
Protein/CRP [HRP]
DCRP00
(3 Reviews)
(83 Publications)
Species: Hu

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FAQs for Themis Antibody (IC6816U) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 IC6816U
Alexa Fluor 405 IC6816V
Alexa Fluor 488 IC6816G
Alexa Fluor 594 IC6816T
Alexa Fluor 647 IC6816R
Alexa Fluor 700 IC6816N
Alexa Fluor 750 IC6816S
Learn about Custom Antibody Labeling

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Bioinformatics Tool for Themis Antibody (IC6816U)

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Bioinformatics

Gene Symbol THEMIS
Uniprot