Themis Antibody (719945) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
719945
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Themis Antibody (719945) [Alexa Fluor® 594] Summary

Immunogen
E. coli-derived recombinant mouse Themis
Lys122-Lys237
Accession # Q8BGW0
Specificity
Detects mouse Themis in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2‑282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2‑282) is observed under reducing conditions.
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
THEMIS
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Themis Antibody (719945) [Alexa Fluor® 594]

  • bA325O24.3
  • bA325O24.4
  • C6orf190
  • C6orf207
  • FLJ40584
  • Gasp
  • GRB2-associated protein
  • MGC163388
  • protein THEMIS
  • signaling phosphoprotein specific for T cells
  • SPOT
  • Themis
  • thymocyte selection associated
  • thymocyte selection pathway associated
  • thymocyte-expressed molecule involved in selection
  • TSEPA

Background

Themis (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, mouse Themis shares 87% and 96% aa sequence identity with human and rat Themis, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Themis Antibody (IC6816T)

Discover related pathways, diseases and genes to Themis Antibody (IC6816T). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Bioinformatics

Gene Symbol THEMIS
Entrez
Uniprot