THEM4 Antibody

Images

 
Western Blot: THEM4 Antibody [NBP1-77360] - Human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: THEM4 Antibody [NBP1-77360] - Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: THEM4 Antibody [NBP1-77360] - Human liver tissue with THEM4 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

THEM4 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4.
Specificity
The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
THEM4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control Peptide
THEM4 Peptide (NBP1-77360PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for THEM4 Antibody

  • C-terminal modulator protein
  • CTMPCarboxyl-terminal modulator protein
  • MGC29636
  • thioesterase superfamily member 4

Background

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56074
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF847
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NBP2-46403
Species: Hu
Applications: IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-33116
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-15071
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-46404
Species: Hu
Applications: IHC, IHC-P
NBP2-24498
Species: Hu, Mu, Rt, Eq, Pm
Applications: WB, IHC-P
NBP2-45745
Species: Hu
Applications: WB, IHC, IF
NB100-56722
Species: Hu, Ca
Applications: WB, B/N, Flow, Func, ICC/IF, IHC-P, IP, Flow-CS
NB110-55290
Species: Hu, Mu, Rt, Po, Ch
Applications: WB, Simple Western, IHC, IHC-P
NBP2-46234
Species: Hu
Applications: WB, IHC
NB100-2357
Species: Hu, Mu, Pm
Applications: WB, IHC-P, IP
NBL1-10259
Species: Hu
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB100-2383
Species: Hu
Applications: WB, ICC/IF
NBP1-77360
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for THEM4 Antibody (NBP1-77360) (0)

There are no publications for THEM4 Antibody (NBP1-77360).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for THEM4 Antibody (NBP1-77360) (0)

There are no reviews for THEM4 Antibody (NBP1-77360). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for THEM4 Antibody (NBP1-77360) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional THEM4 Products

Bioinformatics Tool for THEM4 Antibody (NBP1-77360)

Discover related pathways, diseases and genes to THEM4 Antibody (NBP1-77360). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for THEM4 Antibody (NBP1-77360)

Discover more about diseases related to THEM4 Antibody (NBP1-77360).
 

Pathways for THEM4 Antibody (NBP1-77360)

View related products by pathway.

PTMs for THEM4 Antibody (NBP1-77360)

Learn more about PTMs related to THEM4 Antibody (NBP1-77360).

Blogs on THEM4

There are no specific blogs for THEM4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our THEM4 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol THEM4
Uniprot