THEM4 Antibody - BSA Free

Western Blot: THEM4 Antibody [NBP1-77360] - Human liver tissue lysate with THEM4 antibody at (A) 1 and (B) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: THEM4 Antibody [NBP1-77360] - Immunofluorescence of THEM4 in human liver tissue with THEM4 antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: THEM4 Antibody [NBP1-77360] - Human liver tissue with THEM4 antibody at 2.5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

THEM4 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4. The immunogen is located within amino acids 60 - 110 of THEM4. Amino Acid Squence: EGDSWKRLPSYKRTPTE
• Specificity: The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THEM4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  THEM4 Peptide (NBP1-77360PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for THEM4 Antibody - BSA Free

• C-terminal modulator protein
• CTMPCarboxyl-terminal modulator protein
• MGC29636
• thioesterase superfamily member 4

Background

THEM4, also known as CTMP, binds specifically to the carboxy-terminal regulatory domain of PKB/Akt at the plasma membrane and acts as a negative regulator, reversing the phenotype of v-Akt-transformed cells. Hypermethylation of the THEM4 promoter and transcriptional downregulation of the gene has been reported in multiple glioblastomas, suggesting that epigenetic regulation of THEM4 may play a role in the progression of this cancer. Bioinformatic analysis, confirmed by in vitro testing, indicates that THEM4 is a broad-range, high activity acyl-CoA thioesterase. Recent reports have also indicated that TMEM4 is a mitochondrial protein whose overexpression is associated with an increase in mitochondrial membrane depolarization and caspase-3 and PARP cleavage, suggesting that THEM4 is involved in the apoptotic program. The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THEM4
• Uniprot:
  • NP_444283(Human)