TGN38 Antibody (JF1-024)

Western Blot: TGN38 Antibody (JF1-024) [NBP2-67193] - Analysis of TGN38 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: NIH/3T3 cell lysate Lane 2: Hela cell lysate

Immunohistochemistry-Paraffin: TGN38 Antibody (JF1-024) [NBP2-67193] - Analysis of paraffin-embedded human liver tissue using anti-TGN38 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: TGN38 Antibody (JF1-024) [NBP2-67193] - Analysis of TGN46 on NIH/3T3 cells lysates using anti-TGN46 antibody at 1/1,000 dilution.

Immunohistochemistry-Paraffin: TGN38 Antibody (JF1-024) [NBP2-67193] - Analysis of paraffin-embedded human kidney tissue using anti-TGN46 antibody. Counter stained with hematoxylin.

Product Discontinued

TGN38 Antibody (JF1-024) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human TGN38 aa 43-79 / 437L5L562:L576. (SwissProt: O43493 Human; SwissProt: Q62314 Mouse)
• Localization: Cell membrane, Golgi apparatus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TGOLN2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:50-1:100
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TGN38 Antibody (JF1-024)

• TGN38
• TGN46
• TGN46TGN38 homolog
• TGN48MGC14722
• TGN51
• TGN5148, 51kD isoforms)
• Tgoln1
• Tgoln2
• trans-golgi network protein 2
• Trans-Golgi network protein TGN51
• Ttgn1

Background

TGN38, also known as TGN46, is localized primarily in the Trans-Golgi network. TGN38 has been reported as being the best available marker for human trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGOLN2