TGF-beta RIII Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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TGF-beta RIII Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TGF‑ beta  RIII
Gly23-Thr785
Accession # NP_035708
Specificity
Detects mouse TGF-beta RIII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human TGF‑ beta  sRII and recombinant mouse TGF‑ beta  RII is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TGFBR3
Purity
Immunogen affinity purified
Endotoxin Note
<0.50 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF5034 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RIII Antibody

  • betaglycan proteoglycan
  • Betaglycan
  • BGCAN
  • TBRIII
  • TGF-beta receptor type 3
  • TGF-beta receptor type III
  • TGF-beta RIII
  • TGFbetaRIII
  • TGFBR3
  • TGF-bRIII
  • TGFR-3
  • Transforming growth factor beta receptor III
  • transforming growth factor beta receptor type 3
  • transforming growth factor, beta receptor III (betaglycan, 300kDa)
  • transforming growth factor, beta receptor III

Background

Transforming growth factor beta receptor III (TGF-beta RIII; also betaglycan) is a ubiquitously expressed, 280 kDa type I transmembrane proteoglycan member of the TGF-beta superfamily of proteins (1). Mouse TGF-beta RIII is synthesized as an 850 amino acid (aa) precursor that consists of a 22 aa signal sequence, a 763 extracellular domain (ECD), a 23 aa transmembrane region, and a 42 aa cytoplasmic tail. The large ECD contains heparan sulfate and chondroitin sulfate glycosaminoglycans, five potential N-linked glycosylation sites, and a zona pellucida-like domain from residues 454‑731 (1, 2). The short cytoplasmic domain is rich in serine and threonine, but has no discernible signaling structure typical of receptor kinases (2). Proteolysis at one of two potential juxtamembrane cleavage sites (Lys743Lys and Leu750AlaValVal) allows cells to release TGF-beta RIII in a soluble form (1, 2). Mouse TGF-beta RIII shares 94%, 82%, 80%, and 67% aa sequence identity with rat, human, porcine, and chicken TGF-beta RIII, respectively (2). In all of these species, TGF beta RIII contains 17 cysteines that are 100% conserved (2). TGF-beta RIII binds with high affinity to TGF-beta 1, TGF-beta 2, and TGF-beta 3 isoforms (1). TGF-beta RIII functions by binding, and then "presenting" ligand to TGF-beta type II receptors (1, 3). It also functions to limit ligand availability to the receptor via proteolysis which releases the soluble form of TGF beta RIII along with any bound factors, making them inaccessible to cell-surface receptors (1, 3). TGF-beta RIII can therefore enhance or inhibit cell signaling. TGF-beta RIII has been shown to play an essential role in the formation of the atrioventricular cushion and coronary vessels during development of the heart (4‑6). TGF beta RIII also plays a role in many cancers. Increased expression of TGF beta RIII is found in higher grade lymphomas, and reduced expression of TGF beta RIII is found with advanced stage neuroblastomas and ovarian carcinomas (4, 7‑9). Low TGF-beta RIII expression also correlates with higher grade among a cohort of breast cancers (4, 10). Additionally, overexpression of TGF-beta RIII in MDA-231 human breast cancer cells and DU145 prostate cancer cells results in decreased tumor invasion in vitro and in vivo (4, 11, 12).

  1. Kolodziejczyk, S.M. and B.K. Hall (1996) Biochem. Cell Biol. 74:299.
  2. Ponce-Castaneda, M.V. et al. (1998) Biochim. Biophys. Acta 1384:189.
  3. Lopez-Casillas, F. et al. (1993) Cell 73:1435.
  4. Criswell, T.L. and C.L. Arteaga (2007) J. Biol. Chem. 282:32491.
  5. Brown, C.B. et al. (1999) Science 283:2080.
  6. Compton, L.A. et al. (2007) Circ. Res. 101:784.
  7. Woszczyk, D. et al. (2004) Med. Sci. Monit. 10:CRIII3.
  8. Bristow, R.E. et al. (1999) Cancer 85:658.
  9. Iolascon, A. et al. (2000) Br. J. Cancer 82:1171.
  10. Dong, M. et al. (2007) J. Clin. Invest. 117:206.
  11. Turley, R.S. et al. (2007) Cancer Res. 67:1090.
  12. Sun, L. and  C. Chen (1997) J. Biol. Chem. 272:25367.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TGF-beta RIII Antibody (AF5034)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF5034

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Blogs on TGF-beta RIII.

TGF-beta RIII - a high affinity reservoir for TGF-beta I/II ligands with therapeutic potential
TGF beta (transforming growth factor beta) is a superfamily of cytokines that participate in a variety of cellular processes including growth, proliferation, differentiation, and apoptosis. There are 3 classes of receptors for TGF beta cytokines an...  Read full blog post.

TGF-beta RIII - A multi-functional regulator of the TGF-beta signaling pathway
Transforming growth factor-beta receptor III (TGF-beta RIII) is one of three receptors for the secreted growth factor TGF-beta. Unlike type I and type II TGF-beta receptors, TGF-beta RIII does not participate directly in the propagation of intracel...  Read full blog post.

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Bioinformatics

Gene Symbol TGFBR3
Uniprot