TGF-beta RI/ALK-5 Antibody (854621R) [Alexa Fluor™ Plus 405]

• Reactivity: Hu
• Clone: 854621R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

TGF-beta RI/ALK-5 Antibody (854621R) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human TGF-beta RI/ALK-5 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RI/ALK-5 Antibody (854621R) [Alexa Fluor™ Plus 405]

• AAT5
• activin A receptor type II-like kinase, 53kD
• activin A receptor type II-like kinase, 53kDa
• Activin receptor-like kinase 5
• ACVRLK4
• ALK-5
• ALK-5ALK5
• EC 2.7.11
• EC 2.7.11.30
• LDS1A
• LDS2A
• Serine/threonine-protein kinase receptor R4
• SKR4
• tbetaR-I
• TGFB1R1
• TGF-beta receptor type I
• TGF-beta receptor type-1
• TGF-beta RI
• TGF-beta type I receptor
• TGFbetaRI
• TGFBR1
• TGF-bRI
• TGFR-1
• transforming growth factor beta receptor I
• transforming growth factor, beta receptor 1
• transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)
• Transforming growth factor-beta receptor type I

Background

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase (1, 2). It contains a cysteine-rich extracellular domain (ECD), a transmembrane helix, and a C-terminal cytoplasmic kinase domain (3). Within the cytoplasmic domain there is also a short, conserved regulatory sequence known as the GS region that is N-terminal to the kinase domain (1). Within the ECD, human TGF-beta RI shares 90% and 88% aa sequence identity with mouse and rat TGF-beta RI, respectively. In the presence of TGF-beta , TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII (1). Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3 (2, 4-6). These phosphorylated Smads form heteromeric complexes with Smad4, translocate to the nucleus, and regulate target gene transcription (2, 4-6). TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells (7). Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development (8). Mutations in TGF-beta RI have been identified in pancreatic, colorectal, ovarian, and head and neck cancers (9).

1. Wrana, J.L. et al. (1994) Nature 370:341.
2. Massagué, J. (2012) Nat. Rev. Mol. Cell Biol. 13:616.
3. Huse, M. et al. (1999) Cell 96:425.
4. Marcías-Silva, M. et al. (1996) Cell 87:1215.
5. Zhang, Y. et al. (1996) Nature 383:168.
6. Huse, M. et al. (2001) Mol. Cell 8:671.
7. Larsson, J. et al. (2001) EMBO J. 20:1663.
8. James, J.M. et al. (2013) Development 140:3903.
9. Sheen, Y.Y. et al. (2013) Biomol. Ther. (Seoul) 21:323.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.