TGF-beta RI/ALK-5 Antibody (141231) [Unconjugated]

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

TGFRI and TGFRII association in fibulin-6 KD cells upon TGF-beta stimulus.scr siRNA or fibulin-6 siRNA transfected nCF, after TGF-beta stimulation are subjected to (a) FACS analysis. No difference in the amount of TGF beta RI and TGF beta RII on the surface of control and fibulin-6 KD cells was observed. (b) Immuno-precipitation of TGFRII from cell lysates of scr transfected and TGF-beta stimulated cells, shows more accumulation of TGFRI after western blotting compared to non TGF-beta treated nCF. (c) Immuno-precipitation of TGFRII followed by WB for TGFRI from cell lysates of scr transfected or fibulin-6 KD cells after TGF-beta stimulation display reduced association of receptors in fibulin-6 KD condition. The control input lanes shows no difference and IgG control is also clean (n = 4, p < 0.05). (d) Phosphorylation status of TGFRI was analyzed by western blot using phospho-TGFRI specific antibody. Densitometric analysis of western blot display decreased phosphorylation of TGFRI in fibulin-6 KD cells after TGF-beta stimulation compared to control cells (n = 5, p < 0.01, nonparametric Mann-Whitney U test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28209981), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 141231
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TGF-beta RI/ALK-5 Antibody (141231) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta RI/ALK-5
  Ala21-Glu121
  Accession # BAA05023
• Specificity: Detects human and mouse TGF-beta RI/ALK-5 in Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rrMIS RII, rhTGF‑ beta RII, rhTGF‑ beta RIIb, or rhTGF‑ beta RIII.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TGFBR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta RI/ALK-5 Antibody (141231) [Unconjugated]

• AAT5
• activin A receptor type II-like kinase, 53kD
• activin A receptor type II-like kinase, 53kDa
• Activin receptor-like kinase 5
• ACVRLK4
• ALK-5
• ALK-5ALK5
• EC 2.7.11
• EC 2.7.11.30
• LDS1A
• LDS2A
• Serine/threonine-protein kinase receptor R4
• SKR4
• tbetaR-I
• TGFB1R1
• TGF-beta receptor type I
• TGF-beta receptor type-1
• TGF-beta RI
• TGF-beta type I receptor
• TGFbetaRI
• TGFBR1
• TGF-bRI
• TGFR-1
• transforming growth factor beta receptor I
• transforming growth factor, beta receptor 1
• transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD)
• Transforming growth factor-beta receptor type I

Background

Most cell types express three sizes of receptors for TGF-beta . These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor, originally known as ALK-5 (Activin receptor‑like kinase) is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta . Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

1. Miyazono, K. et al. (1994) Adv. In Immunol. 55:181.
2. Massagùe, J. (1998) Ann. Rev. Biochem. 67:753.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta RI/ALK-5 Antibody (MAB5871)(12)

Publications using MAB5871

• Lei, Z;Tang, R;Wu, Y;Mao, C;Xue, W;Shen, J;Yu, J;Wang, X;Qi, X;Wei, C;Xu, L;Zhu, J;Li, Y;Zhang, X;Ye, C;Chen, X;Yang, X;Zhou, S;Su, C; TGF-?1 induces PD-1 expression in macrophages through SMAD3-STAT3 cooperative signaling in chronic inflammation JCI insight 2024-03-05 [PMID: 38441961] (Flow Cytometry, Mouse)
• Meng Z, Zhang S, Li W et al. Cationic proteins from eosinophils bind bone morphogenetic protein receptors promoting vascular calcification and atherogenesis European heart journal 6/3/2023 [PMID: 37279475]
• Lee, Y;Kim, SH;Jeong, H;Kim, KH;Jeon, D;Cho, Y;Lee, D;Nam, KT; Role of Nox4 in mitigating inflammation and fibrosis in dextran sulfate sodium-induced colitis Cellular and molecular gastroenterology and hepatology 2023-05-17 [PMID: 37207801] (Western Blot, Mouse)
• Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 6/1/2023 [PMID: 36758986]
• Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 2023-06-01 [PMID: 36758986]
• N Ju, H Hayashi, M Shimamura, S Baba, S Yoshida, R Morishita, H Rakugi, H Nakagami Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice Scientific Reports, 2022-07-21;12(1):12474. 2022-07-21 [PMID: 35864207] (ICC, Human)
• Gabitova-Cornell L, Surumbayeva A, Peri S et al. Cholesterol Pathway Inhibition Induces TGF-beta Signaling to Promote Basal Differentiation in Pancreatic Cancer Cancer Cell 9/15/2020 [PMID: 32976774]
• Chowdhury A, Hasselbach L, Echtermeyer F, Jyotsana N, Theilmeier G, Herzog C Fibulin-6 regulates pro-fibrotic TGF-beta responses in neonatal mouse ventricular cardiac fibroblasts. Sci Rep, 2017-02-17;7(0):42725. 2017-02-17 [PMID: 28209981] (Flow Cytometry, Western Blot, Mouse)
• Yin S, Fan Y, Zhang H, Zhao Z, Hao Y, Li J, Sun C, Yang J, Yang Z, Yang X, Lu J, Xi J Differential TGFbeta pathway targeting by miR-122 in humans and mice affects liver cancer metastasis. Nat Commun, 2016-03-18;7(0):11012. 2016-03-18 [PMID: 26987776] (Western Blot, Human)
• O'Brien C, Bonanno L, Zhang H, Wyss-Coray T Beclin 1 regulates neuronal transforming growth factor-beta signaling by mediating recycling of the type I receptor ALK5. Mol Neurodegener, 2015-12-21;10(1):69. 2015-12-21 [PMID: 26692002] (IHC-Fr, Mouse)
• Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65. 2015-02-17 [PMID: 25730867] (Flow Cytometry, Mouse)
• Lamar JM, Iyer V, DiPersio CM Integrin alpha3beta1 potentiates TGFbeta-mediated induction of MMP-9 in immortalized keratinocytes. J. Invest. Dermatol., 2007-08-30;128(3):575-86. 2007-08-30 [PMID: 17762853] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: TGFBR1
• Uniprot:
  • BAA05023()