TFIIB Antibody - BSA Free

Immunoprecipitation: TFIIB Antibody [NBP1-49982] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. GTF2B/ TFIIB was also immunoprecipitated by rabbit anti-TFIIB antibody which recognizes an upstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TFIIB Antibody - BSA Free Summary

• Immunogen: The immunogen for this product maps to a region between residue 256 and 316 of human General Transcription Factor IIB using the numbering given in entry NP_001505.1 (GeneID 2959).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TFIIB Antibody - BSA Free

• general transcription factor IIB
• General transcription factor TFIIB
• S300-II
• TFIIBTF2BRNA polymerase II transcription factor IIB
• transcription initiation factor IIB

Background

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2B