TFIIB Antibodies
We offer TFIIB Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Western Blot. Each TFIIB Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TFIIB Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the TFIIB Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TFIIB polyclonal antibodies and browse our TFIIB monoclonal antibody catalog.
Alternate Names for TFIIB Antibodies
anti-TFIIB antibody, anti-GTF2B antibody, anti-general transcription factor IIB antibody, anti-General transcription factor TFIIB antibody, anti-S300-II antibody, anti-TFIIBTF2BRNA polymerase II transcription factor IIB antibody, anti-transcription initiation factor IIB antibody
12 Results for "tfiib" in Primary Antibodies
![Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining in human gallbladder and pancreas tissues using anti-GTF2B antibody. Corresponding GTF2B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunohistochemistry-Paraffin-NBP2-34143-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining in human gallbladder and pancreas tissues using anti-GTF2B antibody. Corresponding GTF2B RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: TFIIB Antibody [NBP2-34143] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-GTF2B antibody. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Western-Blot-NBP2-34143-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: TFIIB Antibody [NBP2-34143] - Staining of human cell line HEK 293 shows localization to nucleus & nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34143-img0008.jpg)
![Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining of human gallbladder shows high expression.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunohistochemistry-Paraffin-NBP2-34143-img0009.jpg)
![Immunohistochemistry-Paraffin: TFIIB Antibody [NBP2-34143] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunohistochemistry-Paraffin-NBP2-34143-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP3-35718] - Immunofluorescence analysis of L929 cells using TFIIB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35718_rabbit-tfiib-pab-9122024159514.jpg "Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP3-35718] - Immunofluorescence analysis of L929 cells using TFIIB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry: TFIIB Antibody [NBP3-35718] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using TFIIB Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35718_rabbit-tfiib-pab-912202414112889.jpg)
![Immunohistochemistry: TFIIB Antibody [NBP3-35718] - Immunohistochemistry analysis of paraffin-embedded Human colon using TFIIB Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35718_rabbit-tfiib-pab-912202414163040.jpg)
![Immunohistochemistry: TFIIB Antibody [NBP3-35718] - Immunohistochemistry analysis of paraffin-embedded Human esophageal using TFIIB Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35718_rabbit-tfiib-pab-912202415175234.jpg)
![Immunohistochemistry: TFIIB Antibody [NBP3-35718] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using TFIIB Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35718_rabbit-tfiib-pab-912202414201356.jpg)
![Western Blot: TFIIB Antibody [NBP3-35718] - Western blot analysis of various lysates using TFIIB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35718_rabbit-tfiib-pab-91220241581250.jpg)
![Western Blot: TFIIB Antibody [NBP1-32622] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Western-Blot-NBP1-32622-img0012.jpg "Western Blot: TFIIB Antibody [NBP1-32622] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622] - TFIIB antibody detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TFIIB stained by TFIIB antibody diluted at 1:500.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunohistochemistry-Paraffin-NBP1-32622-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: TFIIB Antibody [NBP1-32622] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Western-Blot-NBP1-32622-img0005.jpg)
![Western Blot: TFIIB Antibody [NBP1-32622] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Western-Blot-NBP1-32622-img0011.jpg)
![HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (NBP1-32622) diluted at 1:500 and competitor's antibody (sc-274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-western-blot-26112025185010.jpg)
![TFIIB antibody [N1C3] detects TFIIB protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TFIIB stained by TFIIB antibody [N1C3] (NBP1-32622) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-32622_rabbit-polyclonal-tfiib-antibody-immunocytochemistry-immunofluorescence-2611202518381517.jpg)
![Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Flow cytometric analysis of PFA-fixed HeLa cells. TFIIB antibody (PCRP-GTF2B-1D1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/TFIIB-Antibody-PCRP-GTF2B-1D1-Flow-Cytometry-NBP3-13934-img0001.jpg "Flow Cytometry: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Flow cytometric analysis of PFA-fixed HeLa cells. TFIIB antibody (PCRP-GTF2B-1D1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) [NBP3-13934] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TFIIB Antibody (PCRP-GTF2B-1D1).](http://images.novusbio.com/fullsize/TFIIB-Antibody-PCRP-GTF2B-1D1-Protein-Array-NBP3-13934-img0003.jpg)
![Western Blot: TFIIB Antibody (1H10) [NBP2-45929] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFIIB.](http://images.novusbio.com/fullsize/TFIIB-Antibody-1H10-Western-Blot-NBP2-45929-img0001.jpg "Western Blot: TFIIB Antibody (1H10) [NBP2-45929] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TFIIB.")
![Western Blot: TFIIB Antibody (SR1524) [NBP3-22207] - Western blot analysis using NBP3-22207 in (1) HepG2 cell lysate; (2) RAW264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22207_rabbit-tfiib-mab-sr1524-57202315123423.jpg "Western Blot: TFIIB Antibody (SR1524) [NBP3-22207] - Western blot analysis using NBP3-22207 in (1) HepG2 cell lysate; (2) RAW264.7 cell lysate.")
![Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) - Azide and BSA Free [NBP3-14055] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TFIIB Antibody (PCRP-GTF2B-1D1).](http://images.novusbio.com/fullsize/TFIIB-Antibody-PCRP-GTF2B-1D1-Azide-and-BSA-Free-Protein-Array-NBP3-14055-img0001.jpg "Protein Array: TFIIB Antibody (PCRP-GTF2B-1D1) - Azide and BSA Free [NBP3-14055] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TFIIB Antibody (PCRP-GTF2B-1D1).")
![Immunoprecipitation: TFIIB Antibody [NBP1-49982] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. GTF2B/ TFIIB was also immunoprecipitated by rabbit anti-TFIIB antibody which recognizes an upstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunoprecipitation-NBP1-49982-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: TFIIB Antibody [NBP1-49982] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. GTF2B/ TFIIB was also immunoprecipitated by rabbit anti-TFIIB antibody which recognizes an upstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Immunoprecipitation: TFIIB Antibody [NBP1-49981] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. TFIIB was also immunoprecipitated by rabbit anti- GTF2B/ TFIIB antibody which recognizes a downstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Immunoprecipitation-NBP1-49981-img0002.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: TFIIB Antibody [NBP1-49981] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate. TFIIB was also immunoprecipitated by rabbit anti- GTF2B/ TFIIB antibody which recognizes a downstream epitope. For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![Western Blot: TFIIB Antibody [NBP1-91492] - Titration: 1.0 ug/ml Positive Control: Rat lung.](http://images.novusbio.com/fullsize/TFIIB-Antibody-Western-Blot-NBP1-91492-img0003.jpg "Western Blot: TFIIB Antibody [NBP1-91492] - Titration: 1.0 ug/ml Positive Control: Rat lung.")