TFIIB Antibody

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Western Blot: TFIIB Antibody [NBP1-32622] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:2000. ...read more
Immunocytochemistry/ Immunofluorescence: TFIIB Antibody [NBP1-32622] - TFIIB antibody detects TFIIB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 ...read more
Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622] - TFIIB antibody detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TFIIB stained ...read more
Independent Antibodies: Western Blot: TFIIB Antibody [NBP1-32622] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3]. The ...read more
Western Blot: TFIIB Antibody [NBP1-32622] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit ...read more
Western Blot: TFIIB Antibody [NBP1-32622] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] diluted at 1:1500. The HRP-conjugated ...read more
Western Blot: TFIIB Antibody [NBP1-32622] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (diluted at 1:500.
Western Blot: TFIIB Antibody [NBP1-32622] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody ...read more
Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

TFIIB Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human TFIIB. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (99%), Rat (99%), Xenopus (96%), Zebrafish (95%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GTF2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for TFIIB Antibody

  • general transcription factor IIB
  • General transcription factor TFIIB
  • S300-II
  • TFIIBTF2BRNA polymerase II transcription factor IIB
  • transcription initiation factor IIB

Background

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TFIIB Antibody (NBP1-32622) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for TFIIB Antibody (NBP1-32622)

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Blogs on TFIIB

There are no specific blogs for TFIIB, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol GTF2B