TFIIB Antibody - BSA Free

Western Blot: TFIIB Antibody [NBP1-32622] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: TFIIB Antibody [NBP1-32622] - TFIIB antibody detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TFIIB stained by TFIIB antibody diluted at 1:500.

Western Blot: TFIIB Antibody [NBP1-32622] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TFIIB Antibody [NBP1-32622] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (NBP1-32622) diluted at 1:500 and competitor's antibody (sc-274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with Novus and does not endorse this product.

TFIIB antibody [N1C3] detects TFIIB protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TFIIB stained by TFIIB antibody [N1C3] (NBP1-32622) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10um.

• Reactivity: Hu, Mu, Rt, Bv, RM, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TFIIB Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TFIIB Antibody - BSA Free (NBP1-32622) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TFIIB. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (99%), Rat (99%), Xenopus (96%), Zebrafish (95%), Rhesus Macaque (100%), Bovine (99%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for TFIIB Antibody - BSA Free

• general transcription factor IIB
• General transcription factor TFIIB
• S300-II
• TFIIBTF2BRNA polymerase II transcription factor IIB
• transcription initiation factor IIB

Background

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2B