TEP1 Antibody - BSA Free

Western Blot: TEP1 Antibody [NBP1-77285] - Western blot analysis of TP1 in human kidney tissue lysate with TP1 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: TEP1 Antibody - BSA Free [NBP1-77285] - Immunofluorescence of TP-1 in Human Lung cells with TP-1 antibody at 20 u/mL.

Immunohistochemistry: TEP1 Antibody - BSA Free [NBP1-77285] - Immunohistochemical staining of human lung tissue using TEP1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TEP1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 20 amino acid synthetic peptide from near the amino terminus of human TP1. The immunogen is located within the first 50 amino acids of TP1. Amino Acid Squence: EKLHGHVSAHPDILSLENRC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TEP1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Theoretical MW: 290 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TEP1 Peptide (NBP1-77285PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TEP1 Antibody - BSA Free

• p240telomerase protein component 1
• telomerase-associated protein 1VAULT2
• TLP1p80 telomerase homolog
• TP1Telomerase protein 1
• TROVE domain family, member 1
• TROVE1

Background

Telomerase is an RNA-dependent DNA polymerase that uses an RNA component to add telomeric repeat sequences at the ends of chromosomes. Besides the RNA component which serves as the template that specifies the telomeric repeat, the telomerase complex contains a reverse transcriptase protein (TRT) and various accessory proteins including the telomerase-associated protein 1 (TP1). Telomerase activity is low in most somatic cells, causing the gradual shortening of telomeres which can ultimately lead to telomere fusion and cell death. High levels of telomerase activity are widely seen in cancerous cells and while recent experiments have suggested that telomerase may be a viable target in cancer therapy, expression levels of TP1 do not correlate with malignancy. At least two isoforms of TP1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TEP1 Antibody (NBP1-77285)(1)

Publication using NBP1-77285

• Kurusu R, Fujimoto Y, Morishita H et al. Integrated proteomics identifies p62-dependent selective autophagy of the supramolecular vault complex Developmental cell 2023-05-09 [PMID: 37192622]

Bioinformatics

• Gene Symbol: TEP1
• Uniprot:
  • AAC51107(Human)