Tensin 1 Antibody

Immunohistochemistry-Paraffin: Tensin 1 Antibody [NB100-41087] - Human Kidney at 4ug/ml. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

• Reactivity: Hu, Mu
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Tensin 1 Antibody Summary

• Description: Novus Biologicals Goat Tensin 1 Antibody (NB100-41087) is a polyclonal antibody validated for use in IHC and ELISA. Anti-Tensin 1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-VSKVMLNAGQKR corresponding to C-Terminus according to NP_072174.3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNS1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:2000
• Application Notes: WB: Preliminary experiments in human heart, kidney and skeletal muscle lysates gave no specific signal but low background. IHC-P: Human kidney shows weak staining of the basement membranes of convoluted tubules.

Reactivity Notes

Mouse reactivity reported in scientific literature (Yu M et al).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tensin 1 Antibody

• DKFZp586K0617
• matrix-remodelling associated 6
• Matrix-remodelling-associated protein 6
• MGC88584
• MST091
• MST122
• MST127
• MSTP127
• MXRA6
• tensin 1
• tensin
• tensin-1
• TNSMSTP122

Background

Tensin 1 (TNS1, human Tensin 1 theoretical molecular weight 186kDa) belongs to a family of focal adhesion proteins which in mammals includes three other members: Tensin 2, Tensin 3, and C-terminal tensin-like (Cten or Tensin 4) (1, 2). Tensins localize to focal adhesion sites, which are functional domains of the cellular membrane that mediate interactions between the actin cytoskeleton and the extracellular matrix (1). Transmembrane integrin receptors (alpha-beta heterodimer) predominate within focal adhesions and serve to bridge the interactions between extracellular components and the cytoskeleton (3). Tensin 1 interacts with both actin filaments and beta-integrin receptors within focal adhesion sites to mediate cellular responses to extracellular signals (1, 4). Several common functional domains are shared between some tensin family members including: 1) amino terminal PTEN-related tyrosine phosphatase (PTP) domain, 2) amino terminal actin-binding (ABD-1) domain, 3) amino terminal focal adhesion binding (FAB-N) domain, 4) carboxy terminal Src homology 2 (SH2) domain, 5) carboxy terminal phosphotyrosine binding (PTB) domain, and 6) carboxy terminal focal adhesion binding (FAB-C) domain (4). The PTB domain allows tensins to interact with beta-integrin cytoplasmic tails, while their SH2 domain supports interaction with tyrosine-phosphorylated signaling proteins (1). The interactions of tensins with focal adhesion kinases and phosphatases support diverse cellular processes such as attachment, migration, and polarization. Loss of function studies have revealed that tensin 1 plays a critical role for normal kidney function, skeletal muscle regeneration and angiogenesis (1).

References

1. Lo, S. H. (2017). Tensins. Current Biology. https://doi.org/10.1016/j.cub.2017.02.041

2. Lo, S. H. (2014). C-terminal tensin-like (CTEN): A promising biomarker and target for cancer. International Journal of Biochemistry and Cell Biology. https://doi.org/10.1016/j.biocel.2014.04.003

3. Armitage, S. K., & Plotnikov, S. V. (2016). Bridging the gap: A new study reveals that a protein called talin forms a vital link between microtubules and focal adhesions at the surface of cells. ELife. https://doi.org/10.7554/eLife.19733

4. Lo, S. H. (2006). Focal adhesions: What's new inside. Developmental Biology. https://doi.org/10.1016/j.ydbio.2006.03.029

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tensin 1 Antibody (NB100-41087)(1)

Publication using NB100-41087

• Chen H, Ishii A, Wong WK et al. Molecular characterization of human tensin. Biochem J 2000-10-15 [PMID: 11023826]

Bioinformatics

• Gene Symbol: TNS1
• Entrez:
  • 7145(Human)
  • 21961(Mouse)
  • 301509(Rat)
• Uniprot:
  • NP_072174.3(Human)