Tenascin X Antibody (MTn-12)

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
• Clone: MTn-12
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Tenascin X Antibody (MTn-12) Summary

• Immunogen: Full length native protein (partially purified) (Mouse).
• Epitope: Monoclonal Anti-Mouse Tenascin (rat IgG1 isotype) is derived from the MTn-12 hybridoma1 produced by the fusion of rat myeloma cells and splenocytes from a Lou rat immunized with partially purified mouse tenascin.
• Localization: In immunohistological testing of frozen tissue sections of mouse intestine, the antibody stains the core of the villi but not the epithelial cells.
• Specificity: The antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: TNXB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: Dot: Use at an assay dependant dilution. ELISA: Use at an assay dependant dilution. IF: Use at a dilution of 1/200 for staining of unfixed, frozen tissue sections of mouse intestine. IHC-Fr: Use at an assay dependant dilution. IP: Use at an assay dependant dilution. WB: Use at an assay dependant dilution. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for Tenascin X Antibody (MTn-12)

• Hexabrachion-like protein
• HXBL
• Tenascin X
• tenascin XB
• tenascin XB2
• tenascin-X
• TNX
• TNXB
• TNXB1growth-inhibiting protein 45
• TNXB2tenascin XB1
• TNXBS
• TNXBSTNXTENX
• XB
• XBS
• XB-S
• XBTN-X

Background

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors (Erickson and Bourdon 1989).It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).The tenascin molecule is a disulfide-linked hexamer.The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tenascin X Antibody (NB120-6346)(3)

Publications using NB120-6346

• Yoon SY, Dieterich LC, Tacconi C et al. An important role of podoplanin in hair follicle growth PLoS ONE Jul 23 2019 [PMID: 31335913] (IHC-Fr, IHC, Mouse)
• Aufderheide E amp. Ekblom P Tenascin during gut development: appearance in the mesenchyme, shift in molecular forms, dependence on epithelial-mesenchymal interactions. J Cell Biol 107:2341-9. 1988 [PMID: 2461951]
• Weller A et al. Amino acid sequence of mouse tenascin differential expression of two tenascin isoforms during embryogenesis. J Cell Biol 112:355-62. 1991 [PMID: 1703162]

Bioinformatics

• Gene Symbol: TNXB
• Entrez:
  • 21923(Mouse)