Tenascin C Antibody (578) [Unconjugated]

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Tenascin C was detected in immersion fixed U‑118 MG human glioblastoma/astrocytoma cell line using Rat Anti-Human/Mouse Tenascin C Monoclonal Antibody (Catalog # MAB2138) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, B/N, ICC/IF
Clone
578
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Tenascin C Antibody (578) [Unconjugated] Summary

Immunogen
Mouse immature astrocyte-derived Tenascin C
Specificity
Detect human and mouse Tenascin C in Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
TNC
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry 8-25 ug/mL
  • Neutralization 1 ug/mL
Control
Tenascin C Knockout HeLa Cell Lysate
Reviewed Applications
Read 3 Reviews rated 4
using
MAB2138 in the following applications:

Publications
Read Publications using
MAB2138 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tenascin C Antibody (578) [Unconjugated]

  • 150-225
  • Cytotactin
  • Glioma-associated-extracellular matrix antigen
  • GMEM
  • GP 150-225
  • hexabrachion (tenascin C, cytotactin)
  • hexabrachion (tenascin)
  • Hexabrachion
  • HXB
  • HXBcytotactin
  • JI
  • MGC167029
  • Myotendinous antigen
  • neuronectin
  • Tenascin C
  • Tenascin J1
  • tenascin
  • tenascin-C isoform 14/AD1/16
  • Tenascin-C
  • TNC
  • TN-C
  • TNGP

Background

Tenascin C, also known as hexabrachion, cytotactin, neuronectin, GMEM, JI, myotendinous antigen, glioma-associated-extracellular matrix antigen, and GP 150‑225, is a member of the Tenascin family of extracellular matrix proteins. It is secreted as a disulfide-linked homohexamer whose subunits can vary in size from approximately 200 kDa to over 300 kDa due to differences in glycosylation (1). Rotary-shadowed electron micrographs of the purified molecule show six strands joined to one another at one end in a globular domain with each arm terminating in a knob-like structure (2, 3). The human Tenascin C monomer is synthesized as a precursor with a 22 amino acid (aa) signal sequence and a 2179 aa mature chain. The mature chain consists of a coiled-coil region (aa 118‑145), followed by
15 EGF‑like domains, 15 fibronectin type-III domains, and a fibrinogen C-terminal domain. In addition, there are 23 potential sites of N‑linked glycosylation. Alternative splicing within the fibronectin type-III repeats produces six isoforms for human Tenascin C. Mature human Tenascin C (isoform 1) shares 84% aa sequence identity with mature mouse Tenascin C. In the developing embryo, Tenascin C is expressed during neural, skeletal, and vascular morphogenesis (1, 2). In the adult, it virtually disappears with continued basal expression detectable only in tendon-associated tissues (1, 2). However, great up-regulation in expression occurs in tissues undergoing remodeling processes seen during wound repair and neovascularization or in pathological states such as inflammation or tumorigenesis (1, 4, 5). Biologically, Tenascin C functions as an adhesion-modulatory extracellular matrix protein (1, 4‑8). Specifically, it antagonizes the adhesive effects of fibronectin, and impacts the ability of fibroblasts to deposit and contract the matrix by affecting the morphology and signaling pathways of adherent cells (5‑7). Tenascin C acts by blocking syndecan-4 binding at the edges of the wound and by suppressing fibronectin-mediated activation of RhoA and focal adhesion kinase (FAK) (4‑8). Tenascin C thus promotes epidermal cell migration and proliferation during wound repair.

  1. Hsia, H.C. and J.E. Schwarzbauer (2005) J. Biol. Chem. 280:26641.
  2. Nies, D.E. et al. (1991) J. Biol. Chem. 266:2818.
  3. Erickson, H.P and J.L. Iglesias (1984) Nature 311:267.
  4. Orend, G. et al. (2003) Oncogene 22:3917.
  5. Wenk, M.B. et al. (2000) J. Cell Biol. 150:913.
  6. Midwood, K.S. et al. (2004) Mol. Biol. Cell 15:5670.
  7. Midwood, K.S. and J. E. Schwarzbauer (2002) Mol. Biol. Cell 13:3601.
  8. Hsia, H.C. and J.E. Schwarzbauer (2006) J. Surg. Res. 136:92.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tenascin C Antibody (MAB2138)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
IHC
(3)
IHC-Fr
(1)
IHC-P
(2)
Neutralization
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(6)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB2138 Applications Species
Procopio M, Laszlo C, Al Labban D, Kim D, Bordignon P, Jo S, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski W, Swartz M, Brisken C, Lefort K, Dotto G Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation. Nat Cell Biol, 2015;17(9):1193-204. 2015 [PMID: 26302407] (IHC, Mouse) IHC Mouse
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 2015;194(3):1039-46. 2015 [PMID: 25556244] (Neutralization, Mouse) Neutralization Mouse
Has, Cristina, Kiritsi, Dimitra, Mellerio, Jemima E, Franzke, Claus-We, Wedgeworth, Emma, Tantcheva-Poor, Iliana, Kernland-Lang, Kristin, Itin, Peter, Simpson, Michael, Dopping-Hepenstal, Patricia, Fujimoto, Wataru, McGrath, John A, Bruckner-Tuderman, Leena The missense mutation p.R1303Q in type XVII collagen underlies junctional epidermolysis bullosa resembling Kindler syndrome. J Invest Dermatol, 2014;134(3):845-9. 2014 [PMID: 24005051] (IHC, Human) IHC Human
Grahovac, Jelena, Becker, Dorothea, Wells, Alan Melanoma cell invasiveness is promoted at least in part by the epidermal growth factor-like repeats of tenascin-C. J Invest Dermatol, 2013;133(1):210-20. 2013 [PMID: 22951722] (Western Blot, Human) Western Blot Human
Kern JS, Loeckermann S, Fritsch A, Hausser I, Roth W, Magin TM, Mack C, Muller ML, Paul O, Ruther P, Bruckner-Tuderman L Mechanisms of fibroblast cell therapy for dystrophic epidermolysis bullosa: high stability of collagen VII favors long-term skin integrity. Mol. Ther., 2009;17(9):1605-15. 2009 [PMID: 19568221] (IHC, Mouse) IHC Mouse
Yates CC, Whaley D, Y-Chen A, Kulesekaran P, Hebda PA, Wells A ELR-negative CXC chemokine CXCL11 (IP-9/I-TAC) facilitates dermal and epidermal maturation during wound repair. Am. J. Pathol., 2008;173(3):643-52. 2008 [PMID: 18669615] (IHC-P, Mouse) IHC-P Mouse
Fritsch A, Loeckermann S, Kern JS, Braun A, Bosl MR, Bley TA, Schumann H, von Elverfeldt D, Paul D, Erlacher M, Berens von Rautenfeld D, Hausser I, Fassler R, Bruckner-Tuderman L A hypomorphic mouse model of dystrophic epidermolysis bullosa reveals mechanisms of disease and response to fibroblast therapy. J. Clin. Invest., 2008;118(5):1669-79. 2008 [PMID: 18382769] (IHC-Fr, Mouse) IHC-Fr Mouse
Bujak M, Ren G, Kweon HJ, Dobaczewski M, Reddy A, Taffet G, Wang XF, Frangogiannis NG Essential role of Smad3 in infarct healing and in the pathogenesis of cardiac remodeling. Circulation, 2007;116(19):2127-38. 2007 [PMID: 17967775] (IHC-P, Mouse) IHC-P Mouse

Reviews for Tenascin C Antibody (MAB2138) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using MAB2138:
Filter by Applications
WB
(1)
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Tenascin C MAB2138
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reviewed by:
Anonymous
WB Human 06/17/2019
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Summary

ApplicationWestern Blot
Sample TestedSVG-A immortalized astrocytes
SpeciesHuman
LotKLC0718021
Immunohistochemistry-Frozen Tenascin C MAB2138
Enlarge
reviewed by:
Kirsten Hattermann-Koch
IHC-Fr Mouse 03/11/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotKLC0718021
Immunofluorescence Tenascin C MAB2138
Enlarge
reviewed by:
Andrew White
IF Mouse 12/11/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse skin (hair follicle)
SpeciesMouse

Blocking

Blocking DetailsNormal goat serum, room temperature, 2 hrs

Primary Anitbody

Dilution Ratio1:400

Secondary Antibody

Secondary Descriptiongoat anti rat alexafluor 594

Details

Detection NotesLecia upright microscope
Fixation DetailsFresh frozen sections

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Tenascin C Antibody (MAB2138). (Showing 1 - 1 of 1 FAQs).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

Anonymous
06/17/2019
Application: WB
Species: Human

Kirsten Hattermann-Koch
03/11/2019
Application: IHC-Fr
Species: Mouse

Andrew White
12/11/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol TNC