TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide and BSA Free

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide Free [NBP2-33232] - Analysis of human heart lysates (35ug per lane, RIPA buffer) using TEM8 antibody at 0.3ug/ml. Band observed at 65kDa. (Expected MW of 62.8kDa according to NP_115584.1).

Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide Free [NBP2-33232] - Detection in human liver cancer section using 5 ug/ml concentration of TEM8 antibody. Uniform weak staining was observed in the cancerous cells, while the blood vessels depicted the expected intense positivity for TEM8.

Western Blot: TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide Free [NBP2-33232] - Detection in TEM8 transfected cell lysate using this antibody.

Immunohistochemistry-Paraffin: TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide Free [NBP2-33232] - Formalin-fixed paraffin-embedded section of human colon stained with TEM8 antibody at 2.5 ug/ml concentration using DAB chromogen detection method (10X magnification)

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: 200C1339(SB20)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide and BSA Free Summary

• Immunogen: This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 481-497 (CINFTRVKNNQPAKYPL) of human TEM8.
• Specificity: Nanda et al (2004) note a passage dependent loss of TEM8 in HUVEC suggesting that that TEM8 may be a specific marker of the endothelial cell (EC) phenotype which is lost as primary EC's age in culture and de-differentiate.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANTXR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (Matsuda (2010))
  • Immunohistochemistry 2-5 ug/ml
  • Immunohistochemistry-Paraffin 2-5 ug/ml. Use reported in scientific literature (Hotchkiss et al (2005))
  • Western Blot 1-3 ug/ml
• Application Notes: TEM8 is observed at ~80 kDa in western blots of HUVEC cells (Hotchkiss et al, 2005) and as an 80 kDa and 85 kDa double in human colon cell lysates (Nanada et al, 2004). The size difference is thought to be due to glycosylation as a 70 kDa band was seen when cell extracts in Nanda et al (2004) were treated with a glycosidase cocktail.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TEM8/ANTXR1 Antibody (200C1339(SB20)) - Azide and BSA Free

• anthrax toxin receptor 1
• ANTXR1
• ATR2310008J16Rik
• FLJ10601
• FLJ11298
• FLJ21776
• TEM8
• TEM82810405N18Rik
• Tumor endothelial marker 8

Background

The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANTXR1