TDRKH Antibody (686213)

TDRKH was detected in immersion fixed frozen sections of adult mouse testis using Mouse Anti-Human TDRKH Monoclonal Antibody (Catalog # MAB6286) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of germ cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 686213
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TDRKH Antibody (686213) Summary

• Immunogen: E. coli-derived recombinant human TDRKH
  His206-Leu561
  Accession # Q9Y2W6
• Specificity: Detects human TDRKH in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TDRD1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TDRKH
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TDRKH Antibody (686213)

• TDRD2
• TDRKH
• tudor and KH domain containing
• tudor and KH domain-containing protein

Background

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins.  It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells.  TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome.  Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for noncoding RNA binding.  Human TDRKH is 606 aa in length.  It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412).  There are four potential splice forms.  Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606.  Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH.          

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TDRKH
• Uniprot:
  • Q9Y2W6(Human)