TDRKH Antibody (686213) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: IHC
• Clone: 686213
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

TDRKH Antibody (686213) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human TDRKH
  His206-Leu561
  Accession # Q9Y2W6
• Specificity: Detects human TDRKH in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TDRD1 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TDRKH Antibody (686213) [Alexa Fluor® 405]

• TDRD2
• TDRKH
• tudor and KH domain containing
• tudor and KH domain-containing protein

Background

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins.  It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells.  TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome.  Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for noncoding RNA binding.  Human TDRKH is 606 aa in length.  It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412).  There are four potential splice forms.  Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606.  Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH.          

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.