TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide Free [NBP2-37720] - Analysis (surface staining) of human peripheral blood cells with anti-human TCR Vbeta5.3 related (MEM-262) purified, GAM/APC.

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) - Azide Free [NBP2-37720] - Human peripheral blood lymphocytes stained with MEM-262 FITC and anti-CD3 (UCHT1) APC.

• Reactivity: Hu
• Applications: WB, Flow, Func, IP, CyTOF-ready
• Clone: MEM-262
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free (NB500-520) is a monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human thymoma cell line HPB-ALL.
• Specificity: The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRB
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level is less than 0.01 EU/ug of the protein

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot: this antibody uiniquely recognizes denatured TCR beta chains.
  Functional Application: this antibody activates T cells (V beta 5-related subset)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TCR V beta 5 Antibody (MEM-262) - Azide and BSA Free

• T cell receptor beta locus
• T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
• T-cell receptor, beta cluster
• TCRB
• TRB

Background

The V beta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR V beta5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR beta chain which is expressed on HPB-ALL cells (known expressing TCR V beta5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR V beta5.3 antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRB