TCR V beta 5 Antibody (MEM-262) [Janelia Fluor® 669]

• Reactivity: Hu
• Applications: WB, Flow, IP, CyTOF-ready
• Clone: MEM-262
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 669

TCR V beta 5 Antibody (MEM-262) [Janelia Fluor® 669] Summary

• Immunogen: Human thymoma cell line HPB-ALL.
• Specificity: The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TCR V beta 5 Antibody (MEM-262) [Janelia Fluor® 669]

• T cell receptor beta locus
• T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
• T-cell receptor, beta cluster
• TCRB
• TRB

Background

The V beta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR V beta5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR beta chain which is expressed on HPB-ALL cells (known expressing TCR V beta5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR V beta5.3 antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRB