TCR V beta 5 Antibody (MEM-262) [PE/Cy5.5]

• Reactivity: Hu
• Applications: Flow
• Clone: MEM-262
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE/Cy5.5

TCR V beta 5 Antibody (MEM-262) [PE/Cy5.5] Summary

• Immunogen: Human thymoma cell line HPB-ALL.
• Specificity: The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Alternate Names for TCR V beta 5 Antibody (MEM-262) [PE/Cy5.5]

• T cell receptor beta locus
• T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
• T-cell receptor, beta cluster
• TCRB
• TRB

Background

The V beta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR V beta5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR beta chain which is expressed on HPB-ALL cells (known expressing TCR V beta5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR V beta5.3 antibodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRB