TCR V beta 5 Antibody (MEM-262) - BSA Free

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) [NB500-520] - analysis (surface staining) of human peripheral blood cells with anti-human TCR Vbeta5.3 related (MEM-262) purified, GAM/APC.

Flow Cytometry: TCR V beta 5 Antibody (MEM-262) [NB500-520] - Human peripheral blood lymphocytes stained with MEM-262 FITC and anti-CD3 (UCHT1) APC.

• Reactivity: Hu
• Applications: WB, Flow, IP, CyTOF-ready
• Clone: MEM-262
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TCR V beta 5 Antibody (MEM-262) - BSA Free Summary

• Description: Novus Biologicals Mouse TCR V beta 5 Antibody (MEM-262) - BSA Free (NB500-520) is a monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human thymoma cell line HPB-ALL.
• Specificity: The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Western Blot: Uniquely recognizes denatured TCR beta chains under the conditions. This antibody is CyTOF ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for TCR V beta 5 Antibody (MEM-262) - BSA Free

• T cell receptor beta locus
• T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
• T-cell receptor, beta cluster
• TCRB
• TRB

Background

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vbeta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vbeta5 has been reported to recognize some staphylococcus bacterial superantigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRB
• Entrez:
  • 6957(Human)