TCR V beta 5 Antibody (MEM-262)

Images

 
Flow Cytometry: TCR V beta 5 Antibody (MEM-262) [NB500-520] - Human peripheral blood lymphocytes stained with MEM-262 FITC and anti-CD3 (UCHT1) APC.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IP, CyTOF-ready
Clone
MEM-262
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TCR V beta 5 Antibody (MEM-262) Summary

Immunogen
Human thymoma cell line HPB-ALL.
Specificity
The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
TRB
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Western Blot: Uniquely recognizes denatured TCR beta chains under the conditions

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for TCR V beta 5 Antibody (MEM-262)

  • T cell receptor beta locus
  • T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
  • T-cell receptor, beta cluster
  • TCRB
  • TRB

Background

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vbeta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vbeta5 has been reported to recognize some staphylococcus bacterial superantigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TCR V beta 5 Antibody (NB500-520) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-520AF405
Alexa Fluor 488 Labeled NB500-520AF488
Alexa Fluor 647 Labeled NB500-520AF647
Alexa Fluor 700 Labeled NB500-520AF700
Allophycocyanin Labeled NB500-520APC
DyLight 350 Labeled NB500-520UV
DyLight 405 Labeled NB500-520V
DyLight 488 Labeled NB500-520G
DyLight 680 Labeled NB500-520FR
DyLight 755 Labeled NB500-520IR
PE Labeled NB500-520PE
PerCP Labeled NB500-520PCP

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Bioinformatics

Gene Symbol TRB
Entrez