TCR alpha/beta Antibody (TCR3) [FITC]

Flow Cytometry: TCR alpha/beta Antibody (TCR3) [FITC] [NBP1-28284] - Chicken PBMC were double stained with mouse anti-chicken TCR a/b- FITC and mouse anti-chicken CD3-R-PE. Small lymphocytes were then gated and subjected to 2-color analysis on a Flow cytometer.

• Reactivity: Av, Ch
• Applications: Flow
• Clone: TCR3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

TCR alpha/beta Antibody (TCR3) [FITC] Summary

• Immunogen: CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
• Specificity: Chicken/Peacock/Guinea Fowl
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Reactivity Notes

Chicken

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for TCR alpha/beta Antibody (TCR3) [FITC]

• C)
• D
• FLJ22602
• J
• MGC117436
• MGC22624
• MGC23964
• MGC71411
• T cell receptor alpha locus
• T-cell antigen receptor, alpha polypeptide
• T-cell receptor, alpha (V
• TCRA
• TCRD
• TRA

Background

MAb TCR3 precipitates a CD3-associated heterodimer of Mr 88-kDa (two bands of Mr 48-kDa and 40-kDa upon reduction) on chicken peripheral blood T cells. (1,2) Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 31-kDa from TCR3 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3, (1,2) arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively. (3) The TCR3 MAb reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+ and CD4-CD8- subpopulations. The TCR3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR3+ cells in the blood and spleen express relatively high levels of the TCR3/CD3 receptor complex and are 'single positive, with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (ca. 80% CD4+ vs ca. 20% CD8+). (1,2)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRA