TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

Separation of rat TCR alpha/beta positive thymocytes (red-filled) from TCR alpha/beta negative thymocytes (black-dashed) in Analysis (surface staining) of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 ug/ml) GAM APC.

• Reactivity: Rt, Pm-NHP
• Applications: WB, Flow, Func, IHC, IP, ICC/IF
• Clone: R73
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Low Endotoxin and Azide Free
• Concentration: 1 mg/ml

TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free Summary

• Immunogen: Rat T blasts and erythrocytes
• Specificity: The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-4 ug/ml
  • Functional
  • Immunocytochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Functional application: In vitro activation and in vivo depletion of alpha/beta TCR T cells.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TCR alpha/beta Antibody (R73) - Low Endotoxin and Azide Free

• C)
• D
• FLJ22602
• J
• MGC117436
• MGC22624
• MGC23964
• MGC71411
• T cell receptor alpha locus
• T-cell antigen receptor, alpha polypeptide
• T-cell receptor, alpha (V
• TCRA
• TCRD
• TRA

Background

MAb TCR2 precipitates a CD3-associated heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells. (1) Deglycosylation of the heterodimer yields two polypeptides of Mr 34-kDa and 29-kDa from TCR2 precipitates. In the chicken, two distinct subpopulations of alpha beta T cells appear in the thymus subsequent to the appearance of gamma delta T cells. These subpopulations, originally denoted as TCR2 and TCR3, (1-3) arise sequentially in the thymus during ontogeny and are now known to represent two distinct V beta families, Vbeta1 and Vbeta2, respectively. (4) The TCR2 MAb reacts with approximately 40% of thymocytes, 40-50% of blood mononuclear cells and 40% of splenocytes in the chicken. Two-color immunofluorescence has revealed that most of the TCR2+ thymocytes express both CD4 and CD8 antigens. The TCR2+ cells in blood were found to express either CD4 (74+-2%) or CD8 (26+-4%). TCR2+ cells in the spleen also express either CD4 (37+-1%) or CD8 (64+-4%). Surprisingly, a relatively large subpopulation of CD8+ cells in the spleen are negative for TCR2. (1-3) This observation led to the demonstration that 71+-6% of the TCR1(gamma delta)+ cells in the spleen express the CD8 marker. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRA