TCF7L2 Antibody (6F12-3)

Product Discontinued

TCF7L2 Antibody (6F12-3) Summary

• Immunogen: Produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with a His-tagged fusion protein corresponding to amino acids 31-331 of Human TCF4.
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCF7L2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1-4 ug/ml
• Application Notes: Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Westernblot

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine , 0.15M NaCl and 30% Glycerol
• Preservative: 0.08% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for TCF7L2 Antibody (6F12-3)

• HMG box transcription factor 4
• hTCF-4
• T-cell factor 4
• T-cell factor-4 variant A
• T-cell factor-4 variant B
• T-cell factor-4 variant C
• T-cell factor-4 variant D
• T-cell factor-4 variant E
• T-cell factor-4 variant H
• T-cell factor-4 variant I
• T-cell factor-4 variant J
• T-cell factor-4 variant K
• T-cell factor-4 variant L
• T-cell factor-4 variant M
• T-cell factor-4 variant X2
• T-cell-specific transcription factor 4
• TCF-4T-cell factor-4 variant F
• TCF4T-cell factor-4 variant G
• transcription factor 7-like 2 (T-cell specific, HMG-box)
• transcription factor 7-like 2

Background

T-cell factor 3 and 4 (TCF3 and TCF4) are transcription factors of the High Mobility Group of DNA binding proteins. They are members of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. TCF4 generates transcriptionally active complexes with beta catenin in colon carcinoma cells. TCF3 appears to be expressed independently of TCF4 in the hair follicles, stomach epithelium, and keratinocytes of the skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCF7L2